通过 PhosTag 电泳监测线粒体蛋白质导入机制的蛋白质磷酸化。

4区生物学 Q3 Biochemistry, Genetics and Molecular Biology Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-09-11 DOI:10.1016/bs.mie.2024.07.063

Fatimah Lami Imam, Chris Meisinger, Adinarayana Marada

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引用次数: 0

摘要

线粒体导入机制受几种蛋白激酶的调控，这些蛋白激酶将关键部件磷酸化。这使得蛋白质通量能够根据不断变化的细胞需求进行调整，从而形成动态的细胞器蛋白质组。事实证明，PhosTag 电泳是研究导入机制中这些信号机制的重要工具。

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Monitoring protein phosphorylation at the mitochondrial protein import machinery by PhosTag electrophoresis.

The mitochondrial import machinery is regulated by several protein kinases that phosphorylate key components. This allows an adjustment of the protein flux to changing cellular demands and allow a dynamic organellar proteome. PhosTag electrophoresis has been proven as highly valuably tool to study these signalling machanisms at the import machinery.

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来源期刊

Methods in enzymology 生物-生化研究方法

CiteScore

2.90

自引率

0.00%

发文量

308

审稿时长

3-6 weeks

期刊介绍： The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.