Hankun Su , Yixin Chen , Boya Tang , Fen Xiao , Yuanyuan Sun , Jingjing Chen , Li Deng , Aihua He , Ge Li , Yan Luo , Hui Li
{"title":"用于脊髓损伤修复的天然和生物工程干细胞衍生细胞外囊泡：荟萃分析与试验序列分析。","authors":"Hankun Su , Yixin Chen , Boya Tang , Fen Xiao , Yuanyuan Sun , Jingjing Chen , Li Deng , Aihua He , Ge Li , Yan Luo , Hui Li","doi":"10.1016/j.neuroscience.2024.10.018","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Stem-cell derived extracellular vesicles (EVs) have shown promise in preclinical spinal cord injury (SCI) models but lack a comprehensive literature review for clinical translation guidance.</div></div><div><h3>Methods</h3><div>This <em>meta</em>-analysis with trial sequential analysis systematically search PubMed, Web of Science, Embase, and Cochrane Library databases. Prespecified inclusion criteria were studies reporting on measurable outcomes relevant to SCI repair. Risk of bias and quality of reporting were assessed. Random-effects <em>meta</em>-analyses and subgroup analyses comparing natural and bio-engineered EVs were performed. The study was registered with PROSPERO (CRD42024512122).</div></div><div><h3>Findings</h3><div>The search identified 3935 records, of which 39 studies were included, totaling 1801 animals. Administration of EVs significantly improved locomotor function as measured by Basso-Beattie-Bresnahan or Basso-Mouse-Scale scores at 1 week (natural EVs: SMD 1.50, 95 % CI 1.06–1.95; bio-engineered EVs: SMD 1.93, 95 % CI 1.34–2.52) and 3 weeks (natural EVs: SMD 2.57, 95 % CI 1.96–3.17; bio-engineered EVs: SMD 3.16, 95 % CI 2.29–4.02) post-injury. Subgroup analyses indicated surface modification approaches were most effective among bio-engineered EV strategies. EVs also promoted nerve growth (SMD 2.95, 95 % CI 2.12–3.78), enhanced neuron conductivity (MD 0.75, 95 %CI 0.59–0.90), alleviated inflammation (SMD −3.12, 95 % CI −4.15--2.10), and reduced lesion size (SMD −2.90, 95 % CI −3.87--1.93).</div></div><div><h3>Conclusions</h3><div>Both natural and bio-engineered EVs improve functional and pathological outcomes in animal models of SCI. The enhanced benefits observed with bio-engineered EVs, particularly those utilizing surface modification approaches, highlight the importance of continued exploration into bio-engineering techniques to optimize EVs’ therapeutic efficacy for SCI repair.</div><div><strong>Protocol Registration</strong></div><div>CRD42024512122.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Natural and bio-engineered stem cell-derived extracellular vesicles for spinal cord injury repair: A meta-analysis with trial sequential analysis\",\"authors\":\"Hankun Su , Yixin Chen , Boya Tang , Fen Xiao , Yuanyuan Sun , Jingjing Chen , Li Deng , Aihua He , Ge Li , Yan Luo , Hui Li\",\"doi\":\"10.1016/j.neuroscience.2024.10.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Stem-cell derived extracellular vesicles (EVs) have shown promise in preclinical spinal cord injury (SCI) models but lack a comprehensive literature review for clinical translation guidance.</div></div><div><h3>Methods</h3><div>This <em>meta</em>-analysis with trial sequential analysis systematically search PubMed, Web of Science, Embase, and Cochrane Library databases. Prespecified inclusion criteria were studies reporting on measurable outcomes relevant to SCI repair. Risk of bias and quality of reporting were assessed. Random-effects <em>meta</em>-analyses and subgroup analyses comparing natural and bio-engineered EVs were performed. The study was registered with PROSPERO (CRD42024512122).</div></div><div><h3>Findings</h3><div>The search identified 3935 records, of which 39 studies were included, totaling 1801 animals. Administration of EVs significantly improved locomotor function as measured by Basso-Beattie-Bresnahan or Basso-Mouse-Scale scores at 1 week (natural EVs: SMD 1.50, 95 % CI 1.06–1.95; bio-engineered EVs: SMD 1.93, 95 % CI 1.34–2.52) and 3 weeks (natural EVs: SMD 2.57, 95 % CI 1.96–3.17; bio-engineered EVs: SMD 3.16, 95 % CI 2.29–4.02) post-injury. Subgroup analyses indicated surface modification approaches were most effective among bio-engineered EV strategies. EVs also promoted nerve growth (SMD 2.95, 95 % CI 2.12–3.78), enhanced neuron conductivity (MD 0.75, 95 %CI 0.59–0.90), alleviated inflammation (SMD −3.12, 95 % CI −4.15--2.10), and reduced lesion size (SMD −2.90, 95 % CI −3.87--1.93).</div></div><div><h3>Conclusions</h3><div>Both natural and bio-engineered EVs improve functional and pathological outcomes in animal models of SCI. The enhanced benefits observed with bio-engineered EVs, particularly those utilizing surface modification approaches, highlight the importance of continued exploration into bio-engineering techniques to optimize EVs’ therapeutic efficacy for SCI repair.</div><div><strong>Protocol Registration</strong></div><div>CRD42024512122.</div></div>\",\"PeriodicalId\":19142,\"journal\":{\"name\":\"Neuroscience\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-10-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neuroscience\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0306452224005281\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroscience","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0306452224005281","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
背景：干细胞衍生的细胞外囊泡（EVs干细胞衍生的细胞外囊泡（EVs）在临床前脊髓损伤（SCI）模型中显示出前景，但缺乏全面的文献综述来指导临床转化：这项荟萃分析和试验序列分析系统地检索了 PubMed、Web of Science、Embase 和 Cochrane Library 数据库。预设的纳入标准是报告与 SCI 修复相关的可测量结果的研究。对偏倚风险和报告质量进行了评估。对天然 EV 和生物工程 EV 进行了随机效应荟萃分析和亚组分析。该研究已在 PROSPERO（CRD42024512122）注册：研究结果：检索到 3935 条记录，其中包括 39 项研究，共计 1801 只动物。根据巴索-巴蒂-布雷斯纳汉（Basso-Beattie-Bresnahan）或巴索-小鼠量表（Basso-Mouse-Scale）的评分，服用EVs能明显改善动物1周后的运动功能（天然EVs：SMD 1.50，95 %）：SMD：1.50，95 % CI 1.06-1.95；生物工程EVs：1.93，95 % CI 1.34-2.52）和 3 周（天然 EVs：SMD为2.57，95 % CI为1.96-3.17；生物工程EVs：损伤后 3 周（天然 EV：SMD 2.57，95 % CI 1.96-3.17；生物工程 EV：SMD 3.16，95 % CI 2.29-4.02）。分组分析表明，在生物工程EV策略中，表面修饰方法最为有效。EVs还能促进神经生长（SMD 2.95，95 % CI 2.12-3.78）、增强神经元传导性（MD 0.75，95 %CI 0.59-0.90）、减轻炎症（SMD -3.12，95 % CI -4.15--2.10）并缩小病变范围（SMD -2.90，95 % CI -3.87--1.93）：结论：天然EVs和生物工程EVs都能改善SCI动物模型的功能和病理结果。结论：天然EVs和生物工程EVs都能改善SCI动物模型的功能和病理结果。观察到生物工程EVs，尤其是利用表面修饰方法的EVs，能带来更多益处，这凸显了继续探索生物工程技术以优化EVs对SCI修复疗效的重要性。协议注册号：CRD42024512122。
Natural and bio-engineered stem cell-derived extracellular vesicles for spinal cord injury repair: A meta-analysis with trial sequential analysis
Background
Stem-cell derived extracellular vesicles (EVs) have shown promise in preclinical spinal cord injury (SCI) models but lack a comprehensive literature review for clinical translation guidance.
Methods
This meta-analysis with trial sequential analysis systematically search PubMed, Web of Science, Embase, and Cochrane Library databases. Prespecified inclusion criteria were studies reporting on measurable outcomes relevant to SCI repair. Risk of bias and quality of reporting were assessed. Random-effects meta-analyses and subgroup analyses comparing natural and bio-engineered EVs were performed. The study was registered with PROSPERO (CRD42024512122).
Findings
The search identified 3935 records, of which 39 studies were included, totaling 1801 animals. Administration of EVs significantly improved locomotor function as measured by Basso-Beattie-Bresnahan or Basso-Mouse-Scale scores at 1 week (natural EVs: SMD 1.50, 95 % CI 1.06–1.95; bio-engineered EVs: SMD 1.93, 95 % CI 1.34–2.52) and 3 weeks (natural EVs: SMD 2.57, 95 % CI 1.96–3.17; bio-engineered EVs: SMD 3.16, 95 % CI 2.29–4.02) post-injury. Subgroup analyses indicated surface modification approaches were most effective among bio-engineered EV strategies. EVs also promoted nerve growth (SMD 2.95, 95 % CI 2.12–3.78), enhanced neuron conductivity (MD 0.75, 95 %CI 0.59–0.90), alleviated inflammation (SMD −3.12, 95 % CI −4.15--2.10), and reduced lesion size (SMD −2.90, 95 % CI −3.87--1.93).
Conclusions
Both natural and bio-engineered EVs improve functional and pathological outcomes in animal models of SCI. The enhanced benefits observed with bio-engineered EVs, particularly those utilizing surface modification approaches, highlight the importance of continued exploration into bio-engineering techniques to optimize EVs’ therapeutic efficacy for SCI repair.
期刊介绍：
Neuroscience publishes papers describing the results of original research on any aspect of the scientific study of the nervous system. Any paper, however short, will be considered for publication provided that it reports significant, new and carefully confirmed findings with full experimental details.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
