Yi Shan , Jiayu Lu , Hua Qian , Zhaomin Xia , Xiaoxue Mo , Meidi An , Wen Yang , Siqi Wang , Delu Che , Cheng Wang , Huaizhen He
{"title":"基于细胞膜层析的固定化蛋白质策略及其在发现中药活性物质和毒性物质中的应用","authors":"Yi Shan , Jiayu Lu , Hua Qian , Zhaomin Xia , Xiaoxue Mo , Meidi An , Wen Yang , Siqi Wang , Delu Che , Cheng Wang , Huaizhen He","doi":"10.1016/j.phrs.2024.107492","DOIUrl":null,"url":null,"abstract":"<div><div>Traditional Chinese medicine (TCM) contributes significantly to human health. Owing to the complexity of the ingredients in TCM, it is necessary to conduct basic research on effective substances and identify toxic substances to control the safety of medication. Cell membrane chromatography (CMC) is an important method for identifying target components in complex systems. The cell membrane stationary phase (CMSP) is the core component and key factor in determining the effectiveness of CMC. This review summarizes the development of CMSP with different membrane protein immobilization strategies and the application of CMC in the discovery of active and toxic substances in TCM, with the aim of providing an effective means for the discovery of active ingredients and quality control of TCM.</div></div>","PeriodicalId":19918,"journal":{"name":"Pharmacological research","volume":"210 ","pages":"Article 107492"},"PeriodicalIF":9.1000,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immobilized protein strategies based on cell membrane chromatography and its application in discovering active and toxic substances in traditional Chinese medicine\",\"authors\":\"Yi Shan , Jiayu Lu , Hua Qian , Zhaomin Xia , Xiaoxue Mo , Meidi An , Wen Yang , Siqi Wang , Delu Che , Cheng Wang , Huaizhen He\",\"doi\":\"10.1016/j.phrs.2024.107492\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Traditional Chinese medicine (TCM) contributes significantly to human health. Owing to the complexity of the ingredients in TCM, it is necessary to conduct basic research on effective substances and identify toxic substances to control the safety of medication. Cell membrane chromatography (CMC) is an important method for identifying target components in complex systems. The cell membrane stationary phase (CMSP) is the core component and key factor in determining the effectiveness of CMC. This review summarizes the development of CMSP with different membrane protein immobilization strategies and the application of CMC in the discovery of active and toxic substances in TCM, with the aim of providing an effective means for the discovery of active ingredients and quality control of TCM.</div></div>\",\"PeriodicalId\":19918,\"journal\":{\"name\":\"Pharmacological research\",\"volume\":\"210 \",\"pages\":\"Article 107492\"},\"PeriodicalIF\":9.1000,\"publicationDate\":\"2024-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1043661824004377\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1043661824004377","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Immobilized protein strategies based on cell membrane chromatography and its application in discovering active and toxic substances in traditional Chinese medicine
Traditional Chinese medicine (TCM) contributes significantly to human health. Owing to the complexity of the ingredients in TCM, it is necessary to conduct basic research on effective substances and identify toxic substances to control the safety of medication. Cell membrane chromatography (CMC) is an important method for identifying target components in complex systems. The cell membrane stationary phase (CMSP) is the core component and key factor in determining the effectiveness of CMC. This review summarizes the development of CMSP with different membrane protein immobilization strategies and the application of CMC in the discovery of active and toxic substances in TCM, with the aim of providing an effective means for the discovery of active ingredients and quality control of TCM.
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Pharmacological Research publishes cutting-edge articles in biomedical sciences to cover a broad range of topics that move the pharmacological field forward. Pharmacological research publishes articles on molecular, biochemical, translational, and clinical research (including clinical trials); it is proud of its rapid publication of accepted papers that comprises a dedicated, fast acceptance and publication track for high profile articles.
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