参与黄肉球菌 DK1622 次级代谢调控的意外蛋白质。

IF 3.2 4区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biotechnology and applied biochemistry Pub Date : 2024-11-04 DOI:10.1002/bab.2682
Selar Izzat, Shahlaa M Abdullah, Mohammed N Sabir, Wen-Ping Wei, Bang-Ce Ye, Shwan Rachid
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引用次数: 0

摘要

调节黄腐霉菌中的次级代谢物(SM)有可能影响具有各种生物活性的重要天然产物的形成。本研究的作者先前发现,ROK 失活会影响两种蛋白质(4-羟基苯基丙酮酸二氧酶 [HppD] 和 Hsp90-like 蛋白 [HtpG])的可检测水平。作为证据,本研究旨在阐明这两种蛋白在调节该细菌 SMs 生物合成过程中可能发挥的作用。首先,对相应的基因进行了失活处理,并构建了两个突变株(M. xanthus hppD- 和 htpG-)。随后,对突变体的代谢提取物进行了高效液相色谱-质谱联用分析,结果显示,与野生型相比,突变体产生的几种SM(如DKxanthene、myxalamide A和myxochromide A)显著减少。此外,利用纯化的 ROK 蛋白进行的电泳迁移分析表明,这两种蛋白与编码这两种蛋白的基因启动子区域直接结合。因此可以得出结论,hppD 和 htpG 基因与 SMs 细菌的生物合成调控级联有关,而该级联似乎是由 ROK 蛋白直接调控的。本研究为之前的一项调查提供了更多证据,表明 ROK 对黄杆菌 SMs 的产生具有多重调控作用。
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Unexpected proteins involved in the regulation of secondary metabolism in Myxococcus xanthus DK1622.

Regulating secondary metabolite (SM) in Myxococcus xanthus bears the potential to influence the formation of important natural products with various biological activities. The authors of this study have previously found that the detectable levels of two proteins (4-hydroxyphenylpyruvate dioxygenase [HppD] and a Hsp90-like protein  [HtpG]) are affected by ROK inactivation. As evidence, the current study was designed to elucidate the possible role of these two proteins in regulating the SMs' biosynthesis in this bacterium. To begin with, inactivation of the corresponding genes was carried out, and two mutant strains (M. xanthus hppD- and htpG-) were constructed. Subsequently, high-performance liquid chromatography coupled with mass spectrometry analysis for the metabolic extracts of the mutants revealed a significant reduction in the production of several SMs, like DKxanthene, myxalamide A, and myxochromide A, in comparison to the wild type. Furthermore, electrophoretic mobility shift assays using purified ROK protein suggested a direct binding on the genes' promoter region encoding the two proteins under study. It is therefore possible to conclude that hppD and htpG genes are implicated in the bacterium SMs' biosynthetic regulatory cascade, which seems to be directly regulated by the ROK protein. The present study provides additional evidence to a previous investigation showing the pleiotropic regulatory role of ROK on the production of SMs in M. xanthus.

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来源期刊
Biotechnology and applied biochemistry
Biotechnology and applied biochemistry 工程技术-生化与分子生物学
CiteScore
6.00
自引率
7.10%
发文量
117
审稿时长
3 months
期刊介绍: Published since 1979, Biotechnology and Applied Biochemistry is dedicated to the rapid publication of high quality, significant research at the interface between life sciences and their technological exploitation. The Editors will consider papers for publication based on their novelty and impact as well as their contribution to the advancement of medical biotechnology and industrial biotechnology, covering cutting-edge research in synthetic biology, systems biology, metabolic engineering, bioengineering, biomaterials, biosensing, and nano-biotechnology.
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