欧鼠李提取物在缓解母体分离应激诱导的小鼠自闭症样行为方面的治疗潜力:神经炎症和氧化应激的作用。

IF 6.1 2区 医学 Q1 CHEMISTRY, MEDICINAL Phytotherapy Research Pub Date : 2024-11-04 DOI:10.1002/ptr.8360
Hossein Amini-Khoei, Nafiseh Taei, Hossein Tahmasebi Dehkordi, Zahra Lorigooini, Elham Bijad, Anahita Farahzad, Mohammad Rahimi Madiseh
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引用次数: 0

摘要

自闭症谱系障碍(ASD)是由遗传、环境和表观遗传因素共同作用形成的。多发性硬化症等早期生活压力在这种多方面的神经发育障碍中起着促进作用。这项研究旨在探索欧加马罗勒提取物(O.B.)在减轻雄性小鼠因母体分离(MS)压力而产生的自闭症行为方面的功效,重点研究其对神经炎症和氧化应激的影响。从出生后第 51-53 天到第 58-60 天,用不同剂量(20、40 和 60 毫克/千克)的 O.B. 提取物治疗 MS 小鼠。治疗后进行了行为实验,包括莫里斯水迷宫、三腔试验、穿梭箱和常住者闯入者试验。母体分离的方法是,从 PND 2 到 PND 14,每天将幼仔与母体分离 3 小时。对海马组织进行了分子分析,以评估Toll样受体4(TLR4)、肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的基因表达。对海马和血清丙二醛(MDA)水平以及总抗氧化能力(TAC)进行了测量。通过改善小鼠的空间记忆、被动回避记忆和社会交往,以及减少行为测试中的攻击性,可以证明服用 O.B. 提取物可以改善 MS 小鼠的自闭症样行为。O.B.提取物减轻了氧化应激和神经炎症,表现为MDA水平降低和TAC水平升高,以及海马中TLR4、TNF-α和IL-1β的表达下调。O.B.提取物可能会通过其抗炎和抗氧化特性为自闭症提供一种新的治疗途径。
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Therapeutic Potential of Ocimum basilicum L. Extract in Alleviating Autistic-Like Behaviors Induced by Maternal Separation Stress in Mice: Role of Neuroinflammation and Oxidative Stress.

A confluence of genetic, environmental, and epigenetic factors shapes autism spectrum disorder (ASD). Early-life stressors like MS play a contributing role in this multifaceted neurodevelopmental disorder. This research was to explore the efficacy of Ocimum basilicum L. (O.B.) extract in mitigating behaviors reminiscent of autism prompted by maternal separation (MS) stress in male mice, focusing on its impact on neuroinflammation and oxidative stress. MS mice were treated with O.B. extract at varying dosages (20, 40, and 60 mg/kg) from postnatal days (PND) 51-53 to PND 58-60. Behavioral experiments, including the Morris water maze, three-chamber test, shuttle box, and resident-intruder test, were conducted post-treatment. The method of maternal separation involved separating the pups from their mothers for 3 h daily, from PND 2 to PND 14. Molecular analysis of hippocampal tissue was performed to assess gene expression of Toll-like receptor 4 (TLR4), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β). Hippocampal and serum malondialdehyde (MDA) levels and total antioxidant capacity (TAC) were measured. O.B. extract administration resulted in the amelioration of autistic-like behaviors in MS mice, as evidenced by improved spatial and passive avoidance memories and social interactions, as well as reduced aggression in behavioral tests. O.B. extract attenuated oxidative stress and neuroinflammation, as indicated by decreased MDA and increased TAC levels, as well as downregulation of TLR4, TNF-α, and IL-1β expression in the hippocampus. O.B. extract may offer a novel therapeutic avenue for ASD, potentially mediated through its anti-inflammatory and antioxidant properties.

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来源期刊
Phytotherapy Research
Phytotherapy Research 医学-药学
CiteScore
12.80
自引率
5.60%
发文量
325
审稿时长
2.6 months
期刊介绍: Phytotherapy Research is an internationally recognized pharmacological journal that serves as a trailblazing resource for biochemists, pharmacologists, and toxicologists. We strive to disseminate groundbreaking research on medicinal plants, pushing the boundaries of knowledge and understanding in this field. Our primary focus areas encompass pharmacology, toxicology, and the clinical applications of herbs and natural products in medicine. We actively encourage submissions on the effects of commonly consumed food ingredients and standardized plant extracts. We welcome a range of contributions including original research papers, review articles, and letters. By providing a platform for the latest developments and discoveries in phytotherapy, we aim to support the advancement of scientific knowledge and contribute to the improvement of modern medicine.
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