茄子(Solanum melongena L.)三螺旋基因家族进化分析及非生物胁迫下的表达分析的新发现

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Genomics Pub Date : 2024-11-05 DOI:10.1186/s12864-024-10959-y
Yanhong Lan, Fangyi Gong, Chun Li, Feng Xia, Yifan Li, Xiaojun Liu, Duchen Liu, Genyun Liang, Chao Fang, Peng Cai
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引用次数: 0

摘要

背景:Trihliex转录因子(TFs)在植物生长发育、胁迫响应和植物激素信号网络传递中发挥着重要作用。为了全面研究三螺旋基因在茄子生长发育和非生物胁迫响应中的功能,我们对茄子基因组中的三螺旋基因家族进行了广泛分析:结果:在这项研究中,30个三螺旋基因家族成员不均匀地分布在12条染色体上。根据系统发育关系,这些基因在不同植物物种中是保守的,可分为六个亚家族,同一亚家族中的三螺旋基因具有相似的结构。三螺旋基因的启动子区域含有与植物生长发育、植物激素和非生物胁迫反应有关的顺式作用元件,这表明三螺旋基因有可能应用于开发抗性更强的作物。选择压力评估表明,三螺旋基因经历了纯化选择压力。基于转录组图谱的表达分析表明,SmGT18、SmGT29、SmGT6 和 SmGT28 分别在根、叶、花和果实中高表达。通过实时定量 PCR(qRT-PCR)进行的表达分析表明,大多数三螺旋基因对低温、脱落酸(ABA)和水杨酸(SA)有反应,其中 SmGT29 在冷胁迫条件下表现出显著的上调。随后从茄子中成功克隆了 SmGT29 基因,该基因位于细胞核中,具有强大的转录活性,蛋白质分子量为 74.59 kDa。根据这些发现,推测 SmGT29 是一个关键的候选基因,能积极应对生物胁迫刺激,从而支持植物的先天抗逆机制:总之,本研究首次报道了三螺旋基因及其在茄子植物中的潜在作用。这些结果为提高茄子育种中的抗逆性和品质性状提供了有价值的见解,从而为今后的改良工作提供了重要参考。
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New insights into the evolution analysis of trihelix gene family in eggplant (Solanum melongena L.) and expression analysis under abiotic stress.

Background: Trihliex transcription factors (TFs) play crucial roles in plant growth and development, stress response, and plant hormone signaling network transmission. In order to comprehensively investigate the functions of trihliex genes in eggplant development and the abiotic stress response, we conducted an extensive analysis of the trihliex gene family in the eggplant genome.

Results: In this study, 30 trihelix gene family members were unevenly distributed on 12 chromosomes. On the basis of their phylogenetic relationships, these genes were conserved in different plant species and could be divided into six subfamilies, with trihelix genes within the same subfamily sharing similar structures. The promoter regions of trihelix genes contained cis-acting elements related to plant growth and development, plant hormones, and abiotic stress responses, suggesting potential applications in the development of more resistant crops. Selective pressure assessments indicated that trihliex genes have undergone purifying selection pressure. Expression analysis on the basis of transcriptomic profiles revealed that SmGT18, SmGT29, SmGT6, and SmGT28 are highly expressed in roots, leaves, flowers, and fruits, respectively. Expression analysis via quantitative real-time PCR (qRT‒PCR) revealed that most trihelix genes respond to low temperature, abscisic acid (ABA), and salicylic acid (SA), with SmGT29 exhibiting significant upregulation under cold stress conditions. The SmGT29 gene was subsequently successfully cloned from eggplant, which was located in the nucleus, robust transcriptional activity, and a protein molecular weight of 74.59 kDa. On the basis of these findings, SmGT29 was postulated to be a pivotal candidate gene that actively responds to biotic stress stimuli, thereby supporting the plant's innate stress resistance mechanisms.

Conclusion: In summary, this study was the first report on trihelix genes and their potential roles in eggplant plants. These results provided valuable insights for enhancing stress resistance and quality traits in eggplant breeding, thereby serving as a crucial reference for future improvement efforts.

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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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