超高效液相色谱-四极杆飞行时间串联质谱法快速鉴定体内外三花煎剂的化学成分及大黄蒽醌苷元的代谢物

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL Journal of separation science Pub Date : 2024-11-06 DOI:10.1002/jssc.202400366
Xiang Wang, Jingmei Ma, Qi Qian, Le Gao, Yaqin Zhen, Yurou Tian, Liying Niu, Xinguo Wang
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引用次数: 0

摘要

三花煎是临床治疗中风的经典处方。根据体内外结合的策略,采用超高效液相色谱-四极杆飞行时间串联质谱对三花煎中的化学成分进行了表征,并研究了三花煎中大黄的五种有效蒽醌苷(大黄素、菊花酚、大黄酚、芦荟大黄素、大黄酚)的代谢产物。体外分离和鉴定了 222 种化合物,包括 50 种黄酮类及其苷类、41 种香豆素类、22 种蒽醌类、22 种鞣质类、14 种苯丙类、16 种生物碱类、18 种有机酸及其酯类、14 种木质素类、6 种蒽酮类和 19 种其他化合物。在体内共分离和鉴定出 111 种原型成分。其中,首次在血浆、尿液、粪便和胆汁中分别检测到 26、82、101 和 46 种原型成分和 87 种代谢物。该研究为人参皂苷体内外化学成分的鉴定和潜在药效学成分的分析提供了依据,为进一步研究药效学机制提供了基础。
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Rapid Identification of Chemical Constituents of Sanhua Decoction in Vivo and in Vitro and the Metabolites of Rhubarb Anthraquinone Aglycone by Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Tandem Mass Spectrometry

Sanhua Decoction (SHD) is a classic prescription for the treatment of stroke in the clinic. Based on the combination strategy in vitro and in vivo, the chemical constituents of SHD were characterized by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry, and the metabolites of five effective anthraquinone aglycones (emodin, chrysophanol, rhein, aloe-emodin, and physcion) of rhubarb in SHD were studied. A total of 222 compounds were isolated and identified in vitro, including 50 flavonoids and their glycosides, 41 coumarins, 22 anthraquinones, 22 tannins, 14 phenylpropanoids, 16 alkaloids, 18 organic acids and their esters, 14 lignans, six anthrones, and 19 other compounds. A total of 111 prototype components were isolated and identified in vivo. Among them, 26, 82, 101, and 46 prototype components and 87 metabolites were detected in plasma, urine, feces, and bile for the first time. This study provides a basis for the identification of chemical components in vivo and in vitro and the analysis of potential pharmacodynamic components of SHD, and provides a basis for further study of pharmacodynamic mechanism.

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来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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