FRESH 三维打印唑来膦酸负载壳聚糖/藻酸盐/羟基磷灰石复合热敏水凝胶,用于促进骨再生。

IF 5.3 2区 医学 Q1 PHARMACOLOGY & PHARMACY International Journal of Pharmaceutics Pub Date : 2024-11-03 DOI:10.1016/j.ijpharm.2024.124898
Reem Khaled Wassif , Baher A. Daihom , Mohammed Maniruzzaman
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引用次数: 0

摘要

本研究旨在开发一种用于骨再生的复合热敏水凝胶。这种水凝胶由壳聚糖、藻酸盐和羟基磷灰石组成,并添加了唑来膦酸作为模型药物。研究人员对使用挤压技术三维打印热敏水凝胶的可行性进行了调查。为此采用了名为自由形态可逆嵌入式悬浮水凝胶(FRESH)打印的三维打印技术。为了确定复合水凝胶的特性,进行了多项测试。对凝胶时间、流变特性和体外药物释放进行了分析。此外,还使用 MTT 法评估了复合水凝胶对人骨肉瘤 MG-63 细胞的细胞活力测试。研究结果表明,使用 FRESH 三维打印技术成功打印出了负载唑来膦酸的复合热敏水凝胶,而使用传统的三维打印技术则无法做到这一点。使用扫描电子显微镜对打印结构进行进一步检查后发现,存在多孔和分层结构。经测定,含有和不含 HA 的复合热敏水凝胶的凝胶化时间分别为 10 分钟和 20 分钟,这表明 FRESH 技术在合理的时间内成功地形成了凝胶。研究发现,水凝胶的流动行为是假塑性的,遵循非牛顿流动模式,含羟基磷灰石和不含羟基磷灰石的支架的法罗常数(N)值分别为 1.708 和 1.853。在药物释放方面,含羟基磷灰石和不含羟基磷灰石的支架分别在 360 小时和 48 小时内达到近 100%的唑来膦酸释放量。使用 MTT 法对人骨肉瘤 MG-63 细胞进行的细胞存活率测试表明,复合水凝胶的细胞存活率提高了%,表明该支架具有生物相容性。总之,本研究成功地开发了一种负载唑来膦酸的复合热敏水凝胶,并使用 FRESH 三维打印技术进行了三维打印,用于骨再生应用。这项研究的结果为这种复合水凝胶在未来生物医学应用中的潜在用途提供了宝贵的见解。
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FRESH 3D printing of zoledronic acid-loaded chitosan/alginate/hydroxyapatite composite thermosensitive hydrogel for promoting bone regeneration
The aim of this study was to develop a composite thermosensitive hydrogel for bone regeneration applications. This hydrogel consisted of chitosan, alginate and hydroxyapatite, and was loaded with zoledronic acid as a model drug. The feasibility of three-dimensional (3D) printing of the thermosensitive hydrogel using the extrusion based technique was investigated. The 3D printing technique called Freeform Reversible Embedded Suspended Hydrogel (FRESH) printing was employed for this purpose. To characterize the composite hydrogels, several tests were conducted. The gelation time, rheological properties, and in vitro drug release were analyzed. Additionally, the cell viability test on human osteosarcoma MG-63 cells for the composite hydrogel was assessed using an MTT assay. The results of the study showed that the zoledronic acid-loaded composite thermosensitive hydrogel was successfully printed using the FRESH 3D printing technique which was not possible otherwise i.e., by using traditional 3D printing techniques. Further examination of the printed constructs using a Scanning Electron Microscope revealed the presence of porous and layered structures. The gelation times of the composite thermosensitive hydrogel was determined to be 10 and 20 min, respectively for scaffolds with and without HA, indicating the successful formation of the gel within a reasonable time to the FRESH technique. The flow behavior of the hydrogel was found to be pseudoplastic, following a non-Newtonian flow pattern with Farrow’s constant (N) values of 1.708 and 1.853 for scaffolds with and without hydroxyapatite, respectively. In terms of drug release, scaffolds prepared with and without hydroxyapatite reached nearly 100% of zoledronic acid release in 360 h and 48 h, respectively. The cell viability test on human osteosarcoma MG-63 cells using MTT assay has shown increased cell viability % in the case of composite hydrogel, indicating biocompatibility of the scaffold. Overall, this study successfully developed a composite thermosensitive hydrogel loaded with zoledronic acid for bone regeneration applications and was 3D printed using the FRESH 3D printing technique. The results of this study provide valuable insights into the potential use of this composite hydrogel for future biomedical applications.
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来源期刊
CiteScore
10.70
自引率
8.60%
发文量
951
审稿时长
72 days
期刊介绍: The International Journal of Pharmaceutics is the third most cited journal in the "Pharmacy & Pharmacology" category out of 366 journals, being the true home for pharmaceutical scientists concerned with the physical, chemical and biological properties of devices and delivery systems for drugs, vaccines and biologicals, including their design, manufacture and evaluation. This includes evaluation of the properties of drugs, excipients such as surfactants and polymers and novel materials. The journal has special sections on pharmaceutical nanotechnology and personalized medicines, and publishes research papers, reviews, commentaries and letters to the editor as well as special issues.
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