从一名健康女性捐献者的造血干细胞中生成人类诱导多能干细胞（DMSCi001-A）系

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Stem cell research Pub Date : 2024-10-28 DOI:10.1016/j.scr.2024.103605

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引用次数: 0

摘要

利用无整合外显子载体（包括 OCT3/4/shp53、Sox2/KLF4、L-MYC/LIN28 和 EBNA-1 重编程因子），成功地将从一名 39 岁健康女性体内分离的造血干细胞重编程并转化为诱导多能干细胞（iPSCs）。转化后的 iPSC 株系在无馈源条件下进行培养和扩增。它们显示了正常的核型，表达了多能性标记，并分化成了来自三个生殖层的细胞。这些 iPSCs 能够分化成多种细胞亚型，用于药物发现和机制研究。

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Generation of human induced pluripotent stem cell (DMSCi001-A) line from hematopoietic stem cells of a healthy female donor

Hematopoietic stem cell isolated from a healthy 39-year-old woman were successfully reprogrammed and transformed into induced pluripotent stem cell (iPSCs) by using the integration-free episomal vector included OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28 and EBNA-1 reprogramming factors. The transformed iPSC lines were cultured and expanded under feeder-free condition. They demonstrated the normal karyotype, expressed pluripotency markers and differentiated into cells derived from the three germ layers. These iPSCs are capable of differentiating into numerous cell subtypes for the purposes of drug discovery and mechanism investigation.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.