开发纸基微流体分析装置(μPAD),用于测定水样中的扑热息痛:利用响应曲面法(RSM)进行优化

IF 5.7 3区 环境科学与生态学 Q1 WATER RESOURCES Applied Water Science Pub Date : 2024-11-08 DOI:10.1007/s13201-024-02307-y
Nejat Mohammednur, Ahmed Hussen, Feleke Zewge
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引用次数: 0

摘要

检测和量化各种环境基质中的药物化合物既复杂又具有挑战性。这种困难源于这些化合物的痕量水平,以及缺乏快速、经济、便携的分析方法。为应对这些挑战,本研究旨在开发基于纸张的微流控分析装置(μ-PADs),采用蜂蜡丝网印刷制造。采用响应面方法对反应时间、浓度、试剂量和通道长度等关键参数进行了优化。在 5 ppm 样品浓度、10 μL 试剂量、10 分钟反应时间和 2 cm 通道长度的最佳条件下,对 μPAD 的分析性能进行了评估,并与标准紫外可见分光光度法进行了比较。微流控分析装置的检测限为 0.03 微克/毫升,而紫外可见分光光度计的检测限为 0.01 微克/毫升。虽然本研究中 µ-PAD 的灵敏度(0.03 μg/ml)低于紫外可见分光光度计的灵敏度(0.01 μg/ml),但与之前关于相同分析物的 µ-PAD 报告(1 μg/ml)相比还是有所提高。两种方法的精确度都值得称赞,相对标准偏差均低于 2%。此外,回收率也是可接受和可比较的,µ-PAD 为 86.8%-99.6%,UV-Vis 为 96.5%-99%。分析性能评估表明,µPAD 为痕量扑热息痛分析提供了出色的灵敏度、精确度和准确度。配对 t 检验进一步证实这两种方法之间没有显著的统计学差异,这突出表明 µPADs 具有在没有传统分析仪器的情况下对水样中的痕量扑热息痛进行定量分析的巨大潜力。
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Development of paper-based microfluidic analytical device (μPAD) for the determination of paracetamol in water samples: Optimization using response surface methodology (RSM)

Detecting and quantifying pharmaceutical compounds in various environmental matrices is complex and challenging. This difficulty stems from the trace levels at which these compounds are found and the lack of analytical methods that are rapid, cost-effective, and portable. To address these challenges, this study aimed to develop microfluidic paper-based analytical devices (μ-PADs) using beeswax screen printing for fabrication. Key parameters, including reaction time, concentration, reagent volume, and channel length, were optimized using response surface methodology. Under optimal conditions of 5 ppm sample concentration, 10 μL reagent volume, 10 min reaction time, and 2 cm channel length, the analytical performance of the μPAD was evaluated and compared with the standard UV–Vis spectrophotometry method. The microfluidic analytical device demonstrated detection limits at 0.03 μg/ml, compared to 0.01 μg/ml for the UV–Vis spectrophotometer. Although the sensitivity of µ-PADs in this study (0.03 μg/ml) is lower than that of UV–Vis (0.01 μg/ml), it represents an improvement over the previous µ-PAD report (1 μg/ml) on the same analytes. Both methods exhibited commendable precision, with a relative standard deviation below 2%. Additionally, recovery rates were acceptable and comparable, ranging from 86.8 to 99.6% for µ-PADs and 96.5–99% for UV–Vis. The analytical performance evaluation suggests that µPADs provide excellent sensitivity, precision, and accuracy for trace-level paracetamol analysis. A paired t-test further confirmed no statistically significant difference between the two methods, underscoring the promising potential of µ-PADs for trace-level paracetamol quantification in water samples without conventional analytical instruments.

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来源期刊
Applied Water Science
Applied Water Science WATER RESOURCES-
CiteScore
9.90
自引率
3.60%
发文量
268
审稿时长
13 weeks
期刊介绍:
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