生长微管尖端的力传导分子组合控制着有丝分裂纺锤体的大小

IF 14.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Nature Communications Pub Date : 2024-11-14 DOI:10.1038/s41467-024-54123-2
Lee-Ya Chu, Daniel Stedman, Julian Gannon, Susan Cox, Georgii Pobegalov, Maxim I. Molodtsov
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引用次数: 0

摘要

细胞分裂过程中,正确的有丝分裂纺锤体大小是染色体准确分离的必要条件。它由分子马达和非马达蛋白作用于纺锤体微管所产生的机械力控制。然而,人们对单个蛋白质产生的力如何实现双极纺锤体组织还不甚了解。在这里,我们开发了测量单个分子对这种力平衡的贡献的工具。我们的研究表明,微管正端结合蛋白在微管顶端与负端定向马达协同作用,产生了一个既能产生推力又能产生拉力的系统。为了产生推力,该系统利用了微管尖端生长所产生的力,为力平衡做出了独特的贡献,有别于有丝分裂纺锤体中的所有其他马达。我们的研究结果表明,微管是建立纺锤体大小的基本力发生器,并为了解如何对机械力进行微调以控制染色体分离的保真度铺平了道路。
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Force-transducing molecular ensembles at growing microtubule tips control mitotic spindle size

Correct mitotic spindle size is required for accurate chromosome segregation during cell division. It is controlled by mechanical forces generated by molecular motors and non-motor proteins acting on spindle microtubules. However, how forces generated by individual proteins enable bipolar spindle organization is not well understood. Here, we develop tools to measure contributions of individual molecules to this force balance. We show that microtubule plus-end binding proteins act at microtubule tips synergistically with minus-end directed motors to produce a system that can generate both pushing and pulling forces. To generate pushing force, the system harnesses forces generated by the growing tips of microtubules providing unique contribution to the force balance distinct from all other motors that act in the mitotic spindle. Our results reveal that microtubules are essential force generators for establishing spindle size and pave the way for understanding how mechanical forces can be fine-tuned to control the fidelity of chromosome segregation.

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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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