LNCRNA 1197 通过靶向 miR-206 促进类风湿性关节炎患者纤维母细胞样滑膜细胞的进展：一项试点研究。

IF 1.1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY Annals of clinical and laboratory science Pub Date : 2024-09-01

Xiang Lu, Shanle Yan, Xiaoli Li, Yuan Xue, Liyun Zhang

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引用次数: 0

摘要

目的：研究 LNCRNA 1197 在类风湿关节炎（RA）中的作用及其内在机制：我们研究了 LNCRNA 1197 在类风湿性关节炎（RA）中的作用及其内在机制：我们通过RT-qPCR检测了LNCRNA 1197在RA成纤维细胞样滑膜细胞（RA-FLS）中的表达。方法：我们通过 RTqPCR 检测了 LNCRNA 1197 在 RA 成纤维细胞样滑膜细胞（RA-FLS）中的表达，并通过 Transwell 试验评估了 LNCRNA 对细胞的影响。此外，还进行了 Western 印迹分析以分析增殖相关蛋白的表达，以及流式细胞术和 CCK-8 分析细胞周期和细胞增殖。我们还确定了 LNCRNA 1197 潜在的下游靶 miRNA：结果：LNCRNA 1197在RA-FLS中高表达。结果：LNCRNA 1197 在 RA-FLS 中高表达，当 LNCRNA 1197 被敲除后，RA-FLS 的细胞增殖、迁移和侵袭得到缓解，促炎细胞因子显著下调。有趣的是，LNCRNA 1197被认为以miR-206为靶点，并通过抑制miR-206促进FLS的进展：总而言之，LNCRNA 1197 通过抑制 miR-206 促进了 RA-FLS 的进展。

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LNCRNA 1197 Promotes the Progression of Fibroblast-Like Synovial Cells by Targeting miR-206 in Rheumatoid Arthritis Patients: A Pilot Study.

Objective: We studied the role of LNCRNA 1197 in rheumatoid arthritis (RA) and its underlying mechanism.

Methods: We detected expression of LNCRNA 1197 in RA fibroblast-like synovial cells (RA-FLS) by RT-qPCR. Functional experiments were conducted to assess the impact of LNCRNA on cells as demonstrated by a Transwell assay. In addition, Western blot analysis was conducted to analyze the expression of proliferation-related proteins and flow cytometry and CCK-8 to analyze cell cycle and cell proliferation. We also identified the potential downstream target miRNA of LNCRNA 1197.

Results: LNCRNA 1197 was highly expressed in RA-FLS. When LNCRNA 1197 was knocked down, the cell proliferation, migration, and invasion of RA-FLS were alleviated, and pro-inflammatory cytokines were significantly downregulated. Interestingly, LNCRNA 1197 was indicated to target miR-206 and promoted progression of FLS by inhibiting miR-206.

Conclusion: Taken altogether, LNCRNA 1197 promotes the progression of RA-FLSs by miR-206 inhibition.

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.