{"title":"引起败血症的大肠埃希菌分离株的遗传多样性和抗生素耐药性模式：系统发育分型和 PFGE 分析。","authors":"Mahshid Vakili , Hamidreza Goli , Javad Javidnia , Tahereh Alipour , Majid Eslami","doi":"10.1016/j.diagmicrobio.2024.116586","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>This study aims to analyze clinical isolates of <em>E. coli</em> causing septicemia across various phylogroups utilizing the PFGE method.</div></div><div><h3>Materials and methods</h3><div>A total of 100 clinical isolates were collected. The presence of <em>CTX</em>-<em>M, TEM, SHV, KPC, MBL</em> and <em>OXA</em>-<em>48</em> genes was detected by PCR. Additionally, phylotyping, serotyping, and virulence-typing assay were done by PCR and PFGE methods to investigate the genetic diversity of the isolates.</div></div><div><h3>Results</h3><div>The O1 serotype and the <em>HlyA</em> gene were the most prevalent serotype and virulence gene, respectively. Notably, 34% of the isolates harbored <em>SHV, TEM</em>, and <em>CTX-M-1</em> β-lactamase genes. All isolates showed resistance to amoxicillin and tetracycline, but no resistance to fosfomycin was seen. The most and least common phylotypes, according to PFGE analysis, belonged to phylogroups B2 and B1, respectively.</div></div><div><h3>Conclusion</h3><div>The data offers valuable insights into the genetic diversity and antibiotic resistance patterns of <em>E. coli</em> isolates responsible for septicemia.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 1","pages":"Article 116586"},"PeriodicalIF":2.1000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic diversity and antibiotic resistance patterns of Escherichia coli isolates causing septicemia: A phylogenetic typing and PFGE analysis\",\"authors\":\"Mahshid Vakili , Hamidreza Goli , Javad Javidnia , Tahereh Alipour , Majid Eslami\",\"doi\":\"10.1016/j.diagmicrobio.2024.116586\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><div>This study aims to analyze clinical isolates of <em>E. coli</em> causing septicemia across various phylogroups utilizing the PFGE method.</div></div><div><h3>Materials and methods</h3><div>A total of 100 clinical isolates were collected. The presence of <em>CTX</em>-<em>M, TEM, SHV, KPC, MBL</em> and <em>OXA</em>-<em>48</em> genes was detected by PCR. Additionally, phylotyping, serotyping, and virulence-typing assay were done by PCR and PFGE methods to investigate the genetic diversity of the isolates.</div></div><div><h3>Results</h3><div>The O1 serotype and the <em>HlyA</em> gene were the most prevalent serotype and virulence gene, respectively. Notably, 34% of the isolates harbored <em>SHV, TEM</em>, and <em>CTX-M-1</em> β-lactamase genes. All isolates showed resistance to amoxicillin and tetracycline, but no resistance to fosfomycin was seen. The most and least common phylotypes, according to PFGE analysis, belonged to phylogroups B2 and B1, respectively.</div></div><div><h3>Conclusion</h3><div>The data offers valuable insights into the genetic diversity and antibiotic resistance patterns of <em>E. coli</em> isolates responsible for septicemia.</div></div>\",\"PeriodicalId\":11329,\"journal\":{\"name\":\"Diagnostic microbiology and infectious disease\",\"volume\":\"111 1\",\"pages\":\"Article 116586\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-10-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diagnostic microbiology and infectious disease\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0732889324004115\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diagnostic microbiology and infectious disease","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0732889324004115","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Genetic diversity and antibiotic resistance patterns of Escherichia coli isolates causing septicemia: A phylogenetic typing and PFGE analysis
Introduction
This study aims to analyze clinical isolates of E. coli causing septicemia across various phylogroups utilizing the PFGE method.
Materials and methods
A total of 100 clinical isolates were collected. The presence of CTX-M, TEM, SHV, KPC, MBL and OXA-48 genes was detected by PCR. Additionally, phylotyping, serotyping, and virulence-typing assay were done by PCR and PFGE methods to investigate the genetic diversity of the isolates.
Results
The O1 serotype and the HlyA gene were the most prevalent serotype and virulence gene, respectively. Notably, 34% of the isolates harbored SHV, TEM, and CTX-M-1 β-lactamase genes. All isolates showed resistance to amoxicillin and tetracycline, but no resistance to fosfomycin was seen. The most and least common phylotypes, according to PFGE analysis, belonged to phylogroups B2 and B1, respectively.
Conclusion
The data offers valuable insights into the genetic diversity and antibiotic resistance patterns of E. coli isolates responsible for septicemia.
期刊介绍：
Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.
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