三维打印与热成型和热固化口内矫治器的生物相容性。

IF 4.3 3区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Frontiers in Bioengineering and Biotechnology Pub Date : 2024-10-29 eCollection Date: 2024-01-01 DOI:10.3389/fbioe.2024.1453888
Joanna Weżgowiec, Andrzej Małysa, Wojciech Szlasa, Julita Kulbacka, Agnieszka Chwiłkowska, Marek Ziętek, Mieszko Więckiewicz
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引用次数: 0

摘要

目的:增材制造技术的发展有望彻底改变医疗器械的制造。这种技术(也称为三维打印)提供了精确、经济高效和个性化的方法,尤其有利于口内设备的生产。尽管前景广阔,但有关 3D 打印口内设备生物相容性的研究仍然有限。我们的研究旨在填补这一空白:我们研究了通过三种常用于制造不同口内装置的技术加工的材料的细胞毒性:三维打印(Dental LT Clear)、热成型(Duran adjusted with Durasplint LC)和传统热固化(Villacryl H Plus)。我们还研究了化学或紫外线消毒对这些材料生物相容性的影响。我们通过直接接触试验(MTT 和 LDH 试验)和提取试验(PrestoBlue、DCF 和细胞死亡类型试验)评估了诱导人牙龈成纤维细胞(HGFs)的生物效应。此外,我们还在倒置光学显微镜下观察了细胞形态和迁移率的变化。使用接触式轮廓仪评估了材料的表面粗糙度。统计分析采用双向方差分析法:结果:我们的研究结果表明,所有三种制造技术都会诱导 HGFs 产生轻微的细胞毒性效应,直接接触试验和提取试验都证明了这一点。然而,根据 ISO 10993-5:2009 标准,这些材料可以被认为是无毒的,因为与未经处理的对照组相比,观察到的代谢活性降低始终低于 30%:这项新颖的研究证实,3D 打印技术可以安全地替代传统方法制作口内矫治器。结论:这项新颖的研究证实,3D 打印技术可以安全地替代传统方法制作口内矫治器,但仍需进一步测试评估口内矫治器的长期使用情况。
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Biocompatibility of 3D-printed vs. thermoformed and heat-cured intraoral appliances.

Objectives: The development of additive manufacturing has the potential to revolutionize the fabrication of medical devices. This technology, also known as 3D printing, offers precise, cost-effective, and personalized approaches, which could be particularly beneficial in the production of intraoral appliances. Despite its promise, research on the biocompatibility of 3D-printed intraoral devices is still limited. Our study aims to address this gap.

Methods: We examined the cytotoxicity of materials processed via three techniques commonly used for the fabrication of different intraoral appliances: 3D printing (Dental LT Clear), thermoforming (Duran adjusted with Durasplint LC), and conventional heat-curing (Villacryl H Plus). We also investigated the impact of chemical or UVC disinfection on the biocompatibility of these materials. We assessed the biological effects induced in human gingival fibroblasts (HGFs) through both direct contact tests (MTT and LDH assays) and extract tests (PrestoBlue, DCF, and cell death type assays). Additionally, we observed changes in cellular morphology and migration rate under an inverted light microscope. The surface roughness of materials was evaluated using contact profilometry. Statistical analysis was conducted using two-way analysis of variance.

Results: Our findings suggest that all three fabrication techniques induced a slight cytotoxic effect in HGFs, as evidenced by both direct contact and extract tests. However, these materials could be considered nontoxic according to the ISO 10993-5:2009 norm, as the decrease in metabolic activity observed was always less than 30% compared to the untreated control.

Conclusion: This novel study confirms that 3D printing may be a safe alternative to conventional methods for fabricating intraoral appliances. However, further tests assessing the long-term intraoral usage are still needed.

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来源期刊
Frontiers in Bioengineering and Biotechnology
Frontiers in Bioengineering and Biotechnology Chemical Engineering-Bioengineering
CiteScore
8.30
自引率
5.30%
发文量
2270
审稿时长
12 weeks
期刊介绍: The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs. In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.
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