{"title":"新型序列类型（ST）6758 肺炎克雷伯氏菌的特征及 IncX3 质粒在 bla NDM 传播中的作用。","authors":"Yawen Zhang, Qiao Li, Lirong Li, Hao Guo, Fang He","doi":"10.2147/IDR.S488223","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) has emerged as a significant public health threat, particularly as a superbug responsible for nosocomial infections. In this study, we report a novel sequence type 6758 of <i>K. pneumoniae</i> harboring the <i>bla</i> <sub>NDM-1</sub> gene.</p><p><strong>Material and methods: </strong>Antimicrobial susceptibility testing was conducted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). The complete genome sequence of the strain was determined using the Illumina NovaSeq 6000 platform and long-read MinION sequencer. Genomic features and resistance mechanisms of the strain were further comprehensively analysed using various bioinformatics approaches.</p><p><strong>Results: </strong>Antimicrobial susceptibility testing revealed that this strain exhibited resistance to multiple antimicrobials, including ceftazidime, ceftriaxone, cefazolin, cefepime, imipenem, meropenem, ampicillin/sulbactam, and sulfamethoxazole/trimethoprim. The genome analysis identified sixteen resistance genes. The <i>bla</i> <sub>NDM-1</sub> carbapenemase gene is located on a 47,823 bp IncX3-type plasmid (pNDM-CRKP331). A total of 41 <i>K. pneumoniae</i> strains carrying similar IncX3-type plasmids were retrieved from the NCBI database, representing 20 sequence types (STs) across 11 countries. The most common resistance gene carried by these IncX3-type plasmids is <i>bla</i> <sub>NDM</sub>, and all these plasmids contain only the <i>bla</i> <sub>NDM</sub> gene. The <i>bla</i> <sub>NDM</sub>-carrying IncX3-type plasmids are widely prevalent in <i>K. pneumoniae</i> in China, spanning 15 STs.</p><p><strong>Conclusion: </strong>In summary, our study reports the first genome sequence of an ST 6758 <i>K. pneumoniae</i> strain containing the class B β-lactamase <i>bla</i> <sub>NDM-1</sub> isolated from a clinical sample. Given the global emergence of <i>bla</i> <sub>NDM</sub>, measures should be taken to prevent the spread of these <i>bla</i> <sub>NDM</sub>-carrying IncX3-type plasmids. Our findings contribute to the understanding of the transmission mechanisms of <i>bla</i> <sub>NDM</sub> in <i>K. pneumoniae</i>.</p>","PeriodicalId":13577,"journal":{"name":"Infection and Drug Resistance","volume":"17 ","pages":"4935-4943"},"PeriodicalIF":2.9000,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556245/pdf/","citationCount":"0","resultStr":"{\"title\":\"Characterization of a Novel Sequence Type (ST) 6758 <i>Klebsiella Pneumoniae</i> and the Role of IncX3 Plasmid in the Transmission of <i>bla</i> <sub>NDM</sub>.\",\"authors\":\"Yawen Zhang, Qiao Li, Lirong Li, Hao Guo, Fang He\",\"doi\":\"10.2147/IDR.S488223\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) has emerged as a significant public health threat, particularly as a superbug responsible for nosocomial infections. In this study, we report a novel sequence type 6758 of <i>K. pneumoniae</i> harboring the <i>bla</i> <sub>NDM-1</sub> gene.</p><p><strong>Material and methods: </strong>Antimicrobial susceptibility testing was conducted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). The complete genome sequence of the strain was determined using the Illumina NovaSeq 6000 platform and long-read MinION sequencer. Genomic features and resistance mechanisms of the strain were further comprehensively analysed using various bioinformatics approaches.</p><p><strong>Results: </strong>Antimicrobial susceptibility testing revealed that this strain exhibited resistance to multiple antimicrobials, including ceftazidime, ceftriaxone, cefazolin, cefepime, imipenem, meropenem, ampicillin/sulbactam, and sulfamethoxazole/trimethoprim. The genome analysis identified sixteen resistance genes. The <i>bla</i> <sub>NDM-1</sub> carbapenemase gene is located on a 47,823 bp IncX3-type plasmid (pNDM-CRKP331). A total of 41 <i>K. pneumoniae</i> strains carrying similar IncX3-type plasmids were retrieved from the NCBI database, representing 20 sequence types (STs) across 11 countries. The most common resistance gene carried by these IncX3-type plasmids is <i>bla</i> <sub>NDM</sub>, and all these plasmids contain only the <i>bla</i> <sub>NDM</sub> gene. The <i>bla</i> <sub>NDM</sub>-carrying IncX3-type plasmids are widely prevalent in <i>K. pneumoniae</i> in China, spanning 15 STs.</p><p><strong>Conclusion: </strong>In summary, our study reports the first genome sequence of an ST 6758 <i>K. pneumoniae</i> strain containing the class B β-lactamase <i>bla</i> <sub>NDM-1</sub> isolated from a clinical sample. Given the global emergence of <i>bla</i> <sub>NDM</sub>, measures should be taken to prevent the spread of these <i>bla</i> <sub>NDM</sub>-carrying IncX3-type plasmids. Our findings contribute to the understanding of the transmission mechanisms of <i>bla</i> <sub>NDM</sub> in <i>K. pneumoniae</i>.</p>\",\"PeriodicalId\":13577,\"journal\":{\"name\":\"Infection and Drug Resistance\",\"volume\":\"17 \",\"pages\":\"4935-4943\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-11-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556245/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infection and Drug Resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2147/IDR.S488223\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection and Drug Resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/IDR.S488223","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
摘要
目的：耐碳青霉烯类的肺炎克雷伯氏菌（CRKP）已成为一种严重的公共卫生威胁，尤其是作为一种导致医院内感染的超级细菌。在本研究中，我们报告了一种携带 bla NDM-1 基因的新型肺炎克雷伯菌序列 6758 型：材料和方法：根据临床和实验室标准研究所（CLSI）的指南进行了抗菌药敏感性测试。使用 Illumina NovaSeq 6000 平台和长线程 MinION 测序仪测定了菌株的完整基因组序列。利用各种生物信息学方法进一步全面分析了该菌株的基因组特征和耐药机制：结果：抗菌药物敏感性检测显示，该菌株对多种抗菌药物具有耐药性，包括头孢他啶、头孢曲松、头孢唑啉、头孢吡肟、亚胺培南、美罗培南、氨苄西林/舒巴坦和磺胺甲噁唑/三甲氧苄青霉素。基因组分析确定了 16 个耐药基因。bla NDM-1 碳青霉烯酶基因位于 47,823 bp IncX3 型质粒（pNDM-CRKP331）上。从 NCBI 数据库中共检索到 41 株携带类似 IncX3 型质粒的肺炎克雷伯菌株，代表了 11 个国家的 20 种序列类型（ST）。这些 IncX3 型质粒携带的最常见抗性基因是 bla NDM，所有这些质粒都只含有 bla NDM 基因。携带 bla NDM 的 IncX3 型质粒在中国肺炎克雷伯菌中广泛流行，跨越 15 个 ST：总之，我们的研究首次报道了从临床样本中分离出的一株含有 B 类 β-内酰胺酶 bla NDM-1 的 ST 6758 型肺炎克雷伯菌株的基因组序列。鉴于 bla NDM 在全球范围内的出现，应采取措施防止这些携带 Bla NDM 的 IncX3 型质粒的传播。我们的研究结果有助于了解 bla NDM 在肺炎双球菌中的传播机制。
Characterization of a Novel Sequence Type (ST) 6758 Klebsiella Pneumoniae and the Role of IncX3 Plasmid in the Transmission of blaNDM.
Purpose: Carbapenem-resistant Klebsiella pneumoniae (CRKP) has emerged as a significant public health threat, particularly as a superbug responsible for nosocomial infections. In this study, we report a novel sequence type 6758 of K. pneumoniae harboring the blaNDM-1 gene.
Material and methods: Antimicrobial susceptibility testing was conducted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). The complete genome sequence of the strain was determined using the Illumina NovaSeq 6000 platform and long-read MinION sequencer. Genomic features and resistance mechanisms of the strain were further comprehensively analysed using various bioinformatics approaches.
Results: Antimicrobial susceptibility testing revealed that this strain exhibited resistance to multiple antimicrobials, including ceftazidime, ceftriaxone, cefazolin, cefepime, imipenem, meropenem, ampicillin/sulbactam, and sulfamethoxazole/trimethoprim. The genome analysis identified sixteen resistance genes. The blaNDM-1 carbapenemase gene is located on a 47,823 bp IncX3-type plasmid (pNDM-CRKP331). A total of 41 K. pneumoniae strains carrying similar IncX3-type plasmids were retrieved from the NCBI database, representing 20 sequence types (STs) across 11 countries. The most common resistance gene carried by these IncX3-type plasmids is blaNDM, and all these plasmids contain only the blaNDM gene. The blaNDM-carrying IncX3-type plasmids are widely prevalent in K. pneumoniae in China, spanning 15 STs.
Conclusion: In summary, our study reports the first genome sequence of an ST 6758 K. pneumoniae strain containing the class B β-lactamase blaNDM-1 isolated from a clinical sample. Given the global emergence of blaNDM, measures should be taken to prevent the spread of these blaNDM-carrying IncX3-type plasmids. Our findings contribute to the understanding of the transmission mechanisms of blaNDM in K. pneumoniae.
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An international, peer-reviewed, open access journal that focuses on the optimal treatment of infection (bacterial, fungal and viral) and the development and institution of preventative strategies to minimize the development and spread of resistance.
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