在 I 型纤维中高度表达的肌动蛋白 BDNF 可抑制肌管向 II 型纤维分化。

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Reports Pub Date : 2024-11-12 DOI:10.1007/s11033-024-10044-3

Teng Hu, Yasuro Furuichi, Yasuko Manabe, Kenichiro Yamada, Kengo Katakura, Yuna Aoki, Kun Tang, Takaomi Sakai, Nobuharu L Fujii

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引用次数: 0

摘要

背景：肌纤维大致分为慢速肌纤维（I型）和快速肌纤维（II型）。这两类肌纤维共存于同一骨骼肌组织中，通过纤维类型分布决定骨骼肌组织的收缩和代谢特性：通过分别检测每种纤维类型，我们证实脑源性神经营养因子（BDNF）基因在I型纤维中高表达。当暴露于 BDNF 时，原发性肌管表现出肌球蛋白重链（MyHC）II 的表达减少，而 MyHC II 是 II 型纤维特有的标记蛋白。在再生肌肉组织中过度表达 BDNF 会导致 IIA 型纤维分布减少：我们认为，在I型纤维中高度表达的BDNF会下调肌管中MyHC II的表达，最终抑制IIA型纤维的生成。

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Myokine BDNF highly expressed in Type I fibers inhibits the differentiation of myotubes into Type II fibers.

Background: Myofibers are broadly classified as slow-twitch (Type I) and fast-twitch (Type II) fibers. These two types of myofibers coexist within the same skeletal muscle tissue, determining the contractile and metabolic properties of skeletal muscle tissue by fiber type distribution.

Methods and results: By examining each fiber type separately, we confirmed that brain-derived neurotrophic factor (BDNF) gene is highly expressed in Type I fibers. When exposed to BDNF, primary myotubes exhibited reduced expression of Myosin Heavy Chain (MyHC) II, a marker protein characteristic of Type II fibers. BDNF overexpression in regenerating muscle tissue led to a decrease in the distribution of Type IIA fibers.

Conclusions: We suggest that BDNF highly expressed in Type I fibers downregulates MyHC II expression in myotubes, eventually inhibiting Type IIA fiber generation.

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来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.