Abisola R Isaiah, Laneke Luies, Du Toit Loots, Aurelia A Williams, Maré Vlok, Novel N Chegou, Marceline Tutu van Furth, Martijn van der Kuip, Shayne Mason
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Protocol for unified metabolomics and proteomics analysis of formalin-fixed paraffin-embedded tissue.
The use of archival formalin-fixed paraffin-embedded (FFPE) tissue samples for biochemical analyses is problematic because of the formation of a Schiff base, leading to low protein and metabolite yields during analytical extractions. Here, we overcome this issue using a unified protocol on FFPE tissue for metabolomics and proteomics analyses. Using 20 mg of wet mass tissue, this protocol consistently extracted more than 50 metabolites (across 11 classes of metabolites) and over 900 proteins.
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