{"title":"伊朗东南部比尔詹德肺炎克雷伯氏菌分离物的临床和细菌学特征研究：高病毒表型、生物膜形成、毒力因子和抗生素耐药性。","authors":"Ebrahim Shafaie , Mohammad Hosein Mahdizadeh , Masoumeh Amirian , Parvin Askari , Masoud Yousefi","doi":"10.1016/j.diagmicrobio.2024.116595","DOIUrl":null,"url":null,"abstract":"<div><div>This study assessed antimicrobial susceptibility, biofilm formation, hypervirulence traits, and virulence-related genes in <em>K. pneumoniae</em>. 119 isolates underwent antibiotic susceptibility testing using the Kirby-Bauer method, biofilm assessment through Congo red agar and Microtiter plate assays, PCR for various genes, and the String test for hypermucoviscosity. Clinical characteristics and virulence factors of hypervirulent (hvKP) were compared to classic <em>K. pneumoniae</em> (cKP) strains. Among the isolates, 77.3% were multi-drug resistant (MDR), and 83.2% showed biofilm-forming ability, with a higher MDR incidence in biofilm producers. A significant correlation was found between biofilm formation and the presence of the <em>bla</em><sub>CTX-M15</sub> gene. Genes <em>luxS, mrkA, pgaA</em>, and <em>wzm</em> were significantly related to biofilm production. Three <em>K. pneumoniae</em> (2.5%) were identified as hvKP, with higher prevalence of capsular serotypes K1/K2 and virulence-related genes <em>iuc, rmpA</em>, and <em>rmpA2</em> than cKP. The study underscores the importance of surveillance and stewardship in combating MDR, biofilm-forming, highly virulent <em>K. pneumoniae</em>.</div></div>","PeriodicalId":11329,"journal":{"name":"Diagnostic microbiology and infectious disease","volume":"111 1","pages":"Article 116595"},"PeriodicalIF":2.1000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A study of clinical and bacteriological characteristics of Klebsiella pneumoniae isolates in Birjand, South-East Iran: Hypervirulent phenotype, biofilm formation, virulence factors, and antibiotic resistance\",\"authors\":\"Ebrahim Shafaie , Mohammad Hosein Mahdizadeh , Masoumeh Amirian , Parvin Askari , Masoud Yousefi\",\"doi\":\"10.1016/j.diagmicrobio.2024.116595\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This study assessed antimicrobial susceptibility, biofilm formation, hypervirulence traits, and virulence-related genes in <em>K. pneumoniae</em>. 119 isolates underwent antibiotic susceptibility testing using the Kirby-Bauer method, biofilm assessment through Congo red agar and Microtiter plate assays, PCR for various genes, and the String test for hypermucoviscosity. Clinical characteristics and virulence factors of hypervirulent (hvKP) were compared to classic <em>K. pneumoniae</em> (cKP) strains. Among the isolates, 77.3% were multi-drug resistant (MDR), and 83.2% showed biofilm-forming ability, with a higher MDR incidence in biofilm producers. A significant correlation was found between biofilm formation and the presence of the <em>bla</em><sub>CTX-M15</sub> gene. Genes <em>luxS, mrkA, pgaA</em>, and <em>wzm</em> were significantly related to biofilm production. Three <em>K. pneumoniae</em> (2.5%) were identified as hvKP, with higher prevalence of capsular serotypes K1/K2 and virulence-related genes <em>iuc, rmpA</em>, and <em>rmpA2</em> than cKP. The study underscores the importance of surveillance and stewardship in combating MDR, biofilm-forming, highly virulent <em>K. pneumoniae</em>.</div></div>\",\"PeriodicalId\":11329,\"journal\":{\"name\":\"Diagnostic microbiology and infectious disease\",\"volume\":\"111 1\",\"pages\":\"Article 116595\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-11-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diagnostic microbiology and infectious disease\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0732889324004206\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diagnostic microbiology and infectious disease","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0732889324004206","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
A study of clinical and bacteriological characteristics of Klebsiella pneumoniae isolates in Birjand, South-East Iran: Hypervirulent phenotype, biofilm formation, virulence factors, and antibiotic resistance
This study assessed antimicrobial susceptibility, biofilm formation, hypervirulence traits, and virulence-related genes in K. pneumoniae. 119 isolates underwent antibiotic susceptibility testing using the Kirby-Bauer method, biofilm assessment through Congo red agar and Microtiter plate assays, PCR for various genes, and the String test for hypermucoviscosity. Clinical characteristics and virulence factors of hypervirulent (hvKP) were compared to classic K. pneumoniae (cKP) strains. Among the isolates, 77.3% were multi-drug resistant (MDR), and 83.2% showed biofilm-forming ability, with a higher MDR incidence in biofilm producers. A significant correlation was found between biofilm formation and the presence of the blaCTX-M15 gene. Genes luxS, mrkA, pgaA, and wzm were significantly related to biofilm production. Three K. pneumoniae (2.5%) were identified as hvKP, with higher prevalence of capsular serotypes K1/K2 and virulence-related genes iuc, rmpA, and rmpA2 than cKP. The study underscores the importance of surveillance and stewardship in combating MDR, biofilm-forming, highly virulent K. pneumoniae.
期刊介绍：
Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.
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