质谱法阐明溶液和气相中血红蛋白的构象动力学

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2024-11-18 DOI:10.1021/acs.analchem.4c01439
Julian A Harrison, Janic Gabriel, Adam Pruška, Renato Zenobi
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引用次数: 0

摘要

溶液和气相测量可为生物分子构象动力学提供有价值的见解。通过比较这些实验的数据,可以阐明支配生物分子稳定性的相互作用的性质。在这里,我们使用碰撞诱导解离、碰撞诱导解折、表面诱导解离和温控纳米电喷雾质谱法测量了人、牛和猪血红蛋白在溶液和气相中的稳定性。血红蛋白二聚体和四聚体在溶液和气相中的稳定性并不相关,这可能是由于这些分子表面的正负氨基酸组成不同造成的。具体来说,β 亚基上缺少 Lys-116 会使人类血红蛋白二聚体在气相中更容易解离。然而,Lys-60 的存在使亚基更加坚硬,因此无法像其他血红蛋白一样展开。不同来源的血红蛋白四聚体在气相中的稳定性相似,因为四聚体界面上带电氨基酸的组成没有差异。这些结果突显了温控质谱法和碰撞诱导展开如何阐明蛋白质复合物气相和溶液稳定性差异背后的结构原因。
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Conformational Dynamics of Hemoglobin in Solution and the Gas Phase Elucidated by Mass Spectrometry.

Solution and gas-phase measurements can provide valuable insights into biomolecular conformational dynamics. By comparing the data from such experiments, it is possible to elucidate the nature of the interactions governing a biomolecule's stability. Here, we measured human, bovine, and porcine hemoglobin stability in solution and the gas phase using collision-induced dissociation, collision-induced unfolding, surface-induced dissociation, and temperature-controlled nanoelectrospray mass spectrometry. Hemoglobin dimer and tetramer stability in solution and gas phases did not correlate, likely due to differences in the composition of positive and negative amino acids on the surface of these molecules. Specifically, the absence of Lys-116 on the β-subunit makes it easier for the human hemoglobin dimer to dissociate in the gas phase. However, the presence of Lys-60 makes the subunit more rigid thus it cannot unfold to the same extent as the other hemoglobin. Hemoglobin tetramers of different origins had similar stability in the gas phase, as there was no difference in the composition of charged amino acids at the tetramer interface. These results highlight how temperature-controlled mass spectrometry and collision-induced unfolding can elucidate the structural reasons behind differences in the gas-phase and solution stability of protein complexes.

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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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