Amr J Halawani, Saif Elden B Abdalla, Mahmoud M Habibullah, Ghalia Shamlan, Neil D Avent
{"title":"沙特阿拉伯西南部疟疾流行地区赖特血型等位基因和基因型的调查。","authors":"Amr J Halawani, Saif Elden B Abdalla, Mahmoud M Habibullah, Ghalia Shamlan, Neil D Avent","doi":"10.2147/IJGM.S496346","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Inherited blood disorders as well as malaria are prevalent in southwestern Saudi Arabia. Patients with specific hemoglobinopathies may require frequent blood transfusions. Accordingly, alloimmunization may occur if donors and recipients are incompatible. Therefore, examination of various blood group antigens to provide compatible blood units is essential. Two alleles of the Diego (DI) blood group system, <i>DI*02.03</i> and <i>DI*02.04</i> encode the Wright antigens; Wr<sup>a</sup> and Wr<sup>b</sup>, respectively. Anti-Wr<sup>a</sup> may lead to alloimmunization during transfusion and pregnancy. Furthermore, the Wr<sup>b</sup> antigen may involve in interaction between protein receptors for <i>Plasmodium falciparum</i>. This study aimed to investigate the allele/genotype frequencies of the Wright blood groups in southwestern Saudi blood donors regarding the blood transfusion and assessed the population of Jazan Province for susceptibility of <i>Plasmodium falciparum</i> invasion.</p><p><strong>Materials and methods: </strong>One-hundred-fifty Saudi blood donors were enrolled to this study. DNA was extracted from the blood samples. Primer pairs were designed to capture a single nucleotide variation that distinguishes the Wright alleles. Polymerase chain reaction (PCR) was conducted and followed by standard sequencing.</p><p><strong>Results: </strong>Among the 150 genotyped samples, the only observed allele was <i>DI*02.04</i> (n = 150, 100%). Accordingly, the genotype prevalence of <i>DI*02.04/DI*02.04</i> was accounted for (n = 150, 100%).</p><p><strong>Conclusion: </strong>This study demonstrated the allele frequencies of <i>DI*02.03</i> and <i>DI*02.04</i> of the DI blood group system in Saudi blood donors. The <i>DI*02.04</i> allele was the only allele that was observed. Furthermore, the prevalence of the genotypes was determined and the only observed genotype was <i>DI*02.04/DI*02.04</i>. Interestingly, this study indicates that the Saudi Arabian population living in Jazan Province may be more susceptible to <i>Plasmodium falciparum</i> invasion. Moreover, adding the Wright alleles for the transfusion screening panel is not recommended.</p>","PeriodicalId":14131,"journal":{"name":"International Journal of General Medicine","volume":"17 ","pages":"5175-5180"},"PeriodicalIF":2.1000,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568043/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigation of Wright Blood Group Alleles and Genotypes in Malaria-Endemic Area in Southwestern Saudi Arabia.\",\"authors\":\"Amr J Halawani, Saif Elden B Abdalla, Mahmoud M Habibullah, Ghalia Shamlan, Neil D Avent\",\"doi\":\"10.2147/IJGM.S496346\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Inherited blood disorders as well as malaria are prevalent in southwestern Saudi Arabia. Patients with specific hemoglobinopathies may require frequent blood transfusions. Accordingly, alloimmunization may occur if donors and recipients are incompatible. Therefore, examination of various blood group antigens to provide compatible blood units is essential. Two alleles of the Diego (DI) blood group system, <i>DI*02.03</i> and <i>DI*02.04</i> encode the Wright antigens; Wr<sup>a</sup> and Wr<sup>b</sup>, respectively. Anti-Wr<sup>a</sup> may lead to alloimmunization during transfusion and pregnancy. Furthermore, the Wr<sup>b</sup> antigen may involve in interaction between protein receptors for <i>Plasmodium falciparum</i>. This study aimed to investigate the allele/genotype frequencies of the Wright blood groups in southwestern Saudi blood donors regarding the blood transfusion and assessed the population of Jazan Province for susceptibility of <i>Plasmodium falciparum</i> invasion.</p><p><strong>Materials and methods: </strong>One-hundred-fifty Saudi blood donors were enrolled to this study. DNA was extracted from the blood samples. Primer pairs were designed to capture a single nucleotide variation that distinguishes the Wright alleles. Polymerase chain reaction (PCR) was conducted and followed by standard sequencing.</p><p><strong>Results: </strong>Among the 150 genotyped samples, the only observed allele was <i>DI*02.04</i> (n = 150, 100%). Accordingly, the genotype prevalence of <i>DI*02.04/DI*02.04</i> was accounted for (n = 150, 100%).</p><p><strong>Conclusion: </strong>This study demonstrated the allele frequencies of <i>DI*02.03</i> and <i>DI*02.04</i> of the DI blood group system in Saudi blood donors. The <i>DI*02.04</i> allele was the only allele that was observed. Furthermore, the prevalence of the genotypes was determined and the only observed genotype was <i>DI*02.04/DI*02.04</i>. Interestingly, this study indicates that the Saudi Arabian population living in Jazan Province may be more susceptible to <i>Plasmodium falciparum</i> invasion. Moreover, adding the Wright alleles for the transfusion screening panel is not recommended.</p>\",\"PeriodicalId\":14131,\"journal\":{\"name\":\"International Journal of General Medicine\",\"volume\":\"17 \",\"pages\":\"5175-5180\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-11-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568043/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of General Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2147/IJGM.S496346\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of General Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/IJGM.S496346","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
Investigation of Wright Blood Group Alleles and Genotypes in Malaria-Endemic Area in Southwestern Saudi Arabia.
Purpose: Inherited blood disorders as well as malaria are prevalent in southwestern Saudi Arabia. Patients with specific hemoglobinopathies may require frequent blood transfusions. Accordingly, alloimmunization may occur if donors and recipients are incompatible. Therefore, examination of various blood group antigens to provide compatible blood units is essential. Two alleles of the Diego (DI) blood group system, DI*02.03 and DI*02.04 encode the Wright antigens; Wra and Wrb, respectively. Anti-Wra may lead to alloimmunization during transfusion and pregnancy. Furthermore, the Wrb antigen may involve in interaction between protein receptors for Plasmodium falciparum. This study aimed to investigate the allele/genotype frequencies of the Wright blood groups in southwestern Saudi blood donors regarding the blood transfusion and assessed the population of Jazan Province for susceptibility of Plasmodium falciparum invasion.
Materials and methods: One-hundred-fifty Saudi blood donors were enrolled to this study. DNA was extracted from the blood samples. Primer pairs were designed to capture a single nucleotide variation that distinguishes the Wright alleles. Polymerase chain reaction (PCR) was conducted and followed by standard sequencing.
Results: Among the 150 genotyped samples, the only observed allele was DI*02.04 (n = 150, 100%). Accordingly, the genotype prevalence of DI*02.04/DI*02.04 was accounted for (n = 150, 100%).
Conclusion: This study demonstrated the allele frequencies of DI*02.03 and DI*02.04 of the DI blood group system in Saudi blood donors. The DI*02.04 allele was the only allele that was observed. Furthermore, the prevalence of the genotypes was determined and the only observed genotype was DI*02.04/DI*02.04. Interestingly, this study indicates that the Saudi Arabian population living in Jazan Province may be more susceptible to Plasmodium falciparum invasion. Moreover, adding the Wright alleles for the transfusion screening panel is not recommended.
期刊介绍:
The International Journal of General Medicine is an international, peer-reviewed, open access journal that focuses on general and internal medicine, pathogenesis, epidemiology, diagnosis, monitoring and treatment protocols. The journal is characterized by the rapid reporting of reviews, original research and clinical studies across all disease areas.
A key focus of the journal is the elucidation of disease processes and management protocols resulting in improved outcomes for the patient. Patient perspectives such as satisfaction, quality of life, health literacy and communication and their role in developing new healthcare programs and optimizing clinical outcomes are major areas of interest for the journal.
As of 1st April 2019, the International Journal of General Medicine will no longer consider meta-analyses for publication.