{"title":"糖尿病妊娠胎儿组织的非酶糖基化。脐带提取物葡糖赖氨酸含量的测定。","authors":"A Pollak, H R Salzer, A Lischka, M Hayde","doi":"10.1111/j.1651-2227.1988.tb10687.x","DOIUrl":null,"url":null,"abstract":"<p><p>Non-enzymatic glycation of fetal tissue was studied by determining the glucitollysine content of umbilical cord extracts from twelve infants of diabetic mothers and fourteen infants of healthy, non-diabetic women (controls). The single, glycated amino-acid glycitollysine, which reflects the extent of glycation processes in biological samples, was measured by a standard amino acid ion exchange chromatography followed by reverse phase high pressure liquid chromatography. Infants of diabetic mothers had significantly higher cord glucitollysine levels than infants of control mothers (14.3 + 4.6 vs. 5.5 + 2.1 ng/mg dry tissue; M + SD, p less than 0.001). Moreover, five infants of diabetic mothers with congenital anomalies had strikingly high glucitollysine levels, higher than the mean +4 SD of the controls. We conclude, that non-enzymatic glycation of fetal tissue does occur as a result of an in utero exposure to cumulative glycemia. Major congenital anomalies in diabetic pregnancies are associated with a greater extent of non-enzymatic glycation of umbilical cord tissue.</p>","PeriodicalId":75407,"journal":{"name":"Acta paediatrica Scandinavica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1651-2227.1988.tb10687.x","citationCount":"7","resultStr":"{\"title\":\"Non-enzymatic glycation of fetal tissue in diabetic pregnancy. Estimation of the glucitollysine content of umbilical cord extracts.\",\"authors\":\"A Pollak, H R Salzer, A Lischka, M Hayde\",\"doi\":\"10.1111/j.1651-2227.1988.tb10687.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Non-enzymatic glycation of fetal tissue was studied by determining the glucitollysine content of umbilical cord extracts from twelve infants of diabetic mothers and fourteen infants of healthy, non-diabetic women (controls). The single, glycated amino-acid glycitollysine, which reflects the extent of glycation processes in biological samples, was measured by a standard amino acid ion exchange chromatography followed by reverse phase high pressure liquid chromatography. Infants of diabetic mothers had significantly higher cord glucitollysine levels than infants of control mothers (14.3 + 4.6 vs. 5.5 + 2.1 ng/mg dry tissue; M + SD, p less than 0.001). Moreover, five infants of diabetic mothers with congenital anomalies had strikingly high glucitollysine levels, higher than the mean +4 SD of the controls. We conclude, that non-enzymatic glycation of fetal tissue does occur as a result of an in utero exposure to cumulative glycemia. Major congenital anomalies in diabetic pregnancies are associated with a greater extent of non-enzymatic glycation of umbilical cord tissue.</p>\",\"PeriodicalId\":75407,\"journal\":{\"name\":\"Acta paediatrica Scandinavica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1651-2227.1988.tb10687.x\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta paediatrica Scandinavica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/j.1651-2227.1988.tb10687.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta paediatrica Scandinavica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/j.1651-2227.1988.tb10687.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
摘要
通过测定12名糖尿病母亲的婴儿和14名健康的非糖尿病妇女的婴儿(对照组)的脐带提取物的葡糖赖氨酸含量,研究了胎儿组织的非酶糖基化。通过标准氨基酸离子交换色谱法和反相高压液相色谱法测定了反映生物样品中糖基化过程程度的单糖基化氨基酸glycitolysine。糖尿病母亲的婴儿脐带糖聚赖氨酸水平明显高于对照组母亲的婴儿(14.3 + 4.6 vs 5.5 + 2.1 ng/mg干组织;M + SD, p < 0.001)。此外,5名患有先天性异常的糖尿病母亲所生的婴儿的糖糖赖氨酸水平显著高,高于对照组的平均值+4 SD。我们的结论是,胎儿组织的非酶糖基化确实是由于子宫内暴露于累积血糖而发生的。糖尿病妊娠的主要先天性异常与脐带组织的非酶糖化程度较大有关。
Non-enzymatic glycation of fetal tissue in diabetic pregnancy. Estimation of the glucitollysine content of umbilical cord extracts.
Non-enzymatic glycation of fetal tissue was studied by determining the glucitollysine content of umbilical cord extracts from twelve infants of diabetic mothers and fourteen infants of healthy, non-diabetic women (controls). The single, glycated amino-acid glycitollysine, which reflects the extent of glycation processes in biological samples, was measured by a standard amino acid ion exchange chromatography followed by reverse phase high pressure liquid chromatography. Infants of diabetic mothers had significantly higher cord glucitollysine levels than infants of control mothers (14.3 + 4.6 vs. 5.5 + 2.1 ng/mg dry tissue; M + SD, p less than 0.001). Moreover, five infants of diabetic mothers with congenital anomalies had strikingly high glucitollysine levels, higher than the mean +4 SD of the controls. We conclude, that non-enzymatic glycation of fetal tissue does occur as a result of an in utero exposure to cumulative glycemia. Major congenital anomalies in diabetic pregnancies are associated with a greater extent of non-enzymatic glycation of umbilical cord tissue.