Jaroslaw Cendrowski, Marta Wrobel, Michal Mazur, Bartosz Jary, Ranjana Maurya, Surui Wang, Michal Korostynski, Anna Dziewulska, Maria Rohm, Patryk Kuropka, Natalia Pudelko-Malik, Piotr Mlynarz, Agnieszka Dobrzyn, Anja Zeigerer, Marta Miaczynska
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ESCRT-I deficiency reduced the expression of genes encoding enzymes involved in oxidation of fatty acids and amino acids, such as branched-chain amino acids, and increased the expression of genes encoding glycolytic enzymes. The changes in metabolic gene expression were associated with Warburg effect-like metabolic reprogramming that included intracellular accumulation of lipids, increased glucose/glutamine consumption and lactate production. Moreover, depletion of ESCRT-I components led to expansion of the ER and accumulation of small mitochondria, most of which retained proper potential and performed ATP-linked respiration. Mechanistically, the observed transcriptional reprogramming towards glycolysis in the absence of ESCRT-I occurred due to activation of the canonical NFκB and JNK signaling pathways and at least in part by perturbed lysosomal degradation. We propose that by activating the stress signaling pathways ESCRT-I deficiency leads to preferential usage of extracellular nutrients, like glucose and glutamine, for energy production instead of lysosome-derived nutrients, such as fatty acids and branched-chain amino acids.</p>","PeriodicalId":10007,"journal":{"name":"Cellular and Molecular Life Sciences","volume":"81 1","pages":"458"},"PeriodicalIF":6.2000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576715/pdf/","citationCount":"0","resultStr":"{\"title\":\"NFκB and JNK pathways mediate metabolic adaptation upon ESCRT-I deficiency.\",\"authors\":\"Jaroslaw Cendrowski, Marta Wrobel, Michal Mazur, Bartosz Jary, Ranjana Maurya, Surui Wang, Michal Korostynski, Anna Dziewulska, Maria Rohm, Patryk Kuropka, Natalia Pudelko-Malik, Piotr Mlynarz, Agnieszka Dobrzyn, Anja Zeigerer, Marta Miaczynska\",\"doi\":\"10.1007/s00018-024-05490-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Endosomal Sorting Complexes Required for Transport (ESCRTs) are crucial for delivering membrane receptors or intracellular organelles for lysosomal degradation which provides the cell with lysosome-derived nutrients. Yet, how ESCRT dysfunction affects cell metabolism remained elusive. To address this, we analyzed transcriptomes of cells lacking TSG101 or VPS28 proteins, components of ESCRT-I subcomplex. ESCRT-I deficiency reduced the expression of genes encoding enzymes involved in oxidation of fatty acids and amino acids, such as branched-chain amino acids, and increased the expression of genes encoding glycolytic enzymes. The changes in metabolic gene expression were associated with Warburg effect-like metabolic reprogramming that included intracellular accumulation of lipids, increased glucose/glutamine consumption and lactate production. Moreover, depletion of ESCRT-I components led to expansion of the ER and accumulation of small mitochondria, most of which retained proper potential and performed ATP-linked respiration. Mechanistically, the observed transcriptional reprogramming towards glycolysis in the absence of ESCRT-I occurred due to activation of the canonical NFκB and JNK signaling pathways and at least in part by perturbed lysosomal degradation. 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NFκB and JNK pathways mediate metabolic adaptation upon ESCRT-I deficiency.
Endosomal Sorting Complexes Required for Transport (ESCRTs) are crucial for delivering membrane receptors or intracellular organelles for lysosomal degradation which provides the cell with lysosome-derived nutrients. Yet, how ESCRT dysfunction affects cell metabolism remained elusive. To address this, we analyzed transcriptomes of cells lacking TSG101 or VPS28 proteins, components of ESCRT-I subcomplex. ESCRT-I deficiency reduced the expression of genes encoding enzymes involved in oxidation of fatty acids and amino acids, such as branched-chain amino acids, and increased the expression of genes encoding glycolytic enzymes. The changes in metabolic gene expression were associated with Warburg effect-like metabolic reprogramming that included intracellular accumulation of lipids, increased glucose/glutamine consumption and lactate production. Moreover, depletion of ESCRT-I components led to expansion of the ER and accumulation of small mitochondria, most of which retained proper potential and performed ATP-linked respiration. Mechanistically, the observed transcriptional reprogramming towards glycolysis in the absence of ESCRT-I occurred due to activation of the canonical NFκB and JNK signaling pathways and at least in part by perturbed lysosomal degradation. We propose that by activating the stress signaling pathways ESCRT-I deficiency leads to preferential usage of extracellular nutrients, like glucose and glutamine, for energy production instead of lysosome-derived nutrients, such as fatty acids and branched-chain amino acids.
期刊介绍:
Journal Name: Cellular and Molecular Life Sciences (CMLS)
Location: Basel, Switzerland
Focus:
Multidisciplinary journal
Publishes research articles, reviews, multi-author reviews, and visions & reflections articles
Coverage:
Latest aspects of biological and biomedical research
Areas include:
Biochemistry and molecular biology
Cell biology
Molecular and cellular aspects of biomedicine
Neuroscience
Pharmacology
Immunology
Additional Features:
Welcomes comments on any article published in CMLS
Accepts suggestions for topics to be covered