对猪热应激诱导的肠道炎症进行转录组分析的启示和意义

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY BMC Genomics Pub Date : 2024-11-19 DOI:10.1186/s12864-024-10928-5
Zhichao Yu, Yanhong Yong, Xiaoxi Liu, Xingbin Ma, A M Abd El-Aty, Leling Li, Ziyuan Zhong, Xingyi Ye, Xianghong Ju
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引用次数: 0

摘要

背景:热应激(HS)会影响动物的生理和新陈代谢。HS 引起的猪肠道炎症是一种常见疾病,可导致严重腹泻,给养猪业造成巨大经济损失,但该病的分子机制和致病性尚不完全清楚。本研究的目的是鉴定恒定(1、7 和 14 天)HS 条件下猪结肠组织中与炎症相关的差异表达基因(DEGs)和长非编码 RNAs(DELs):结果:构建了 LncRNA 和靶基因相互作用网络。结果:构建了 LncRNA 和靶向基因相互作用网络,随后进行了 GO 注释和 KEGG 通路分析,以确定 DEGs 和 DELs 的功能。结果显示,在 CON/H01、CON/H07 和 CON/H14 组中,DEGs 分别为 57、212 和 54 个,DELs 分别为 87、79 和 55 个。KRT85、CLDN1、S100A12、TM7SF2、CCN1、NR4A1和几个lncRNA可能参与调控肠道炎症的发展。GO分析表明,DEGs和DELs富集于一系列涉及先天性免疫反应、RAGE受体结合以及ERK1和ERK2级联正调控的生物过程中。与炎症相关的 KEGG 通路,如紧密连接(TJ)和 MAPK 信号通路,也富集在 DEGs 和 DELs 中:本研究拓展了HS诱导猪结肠炎症相关基因和lncRNA生物学的知识;分析了HS诱导肠道炎症的lncRNA-mRNA相互作用。这些结果可为我们了解猪肠道对HS反应的分子机制提供一些参考。
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Insights and implications for transcriptomic analysis of heat stress-induced intestinal inflammation in pigs.

Background: Heat stress (HS) can affect the physiology and metabolism of animals. HS-induced intestinal inflammation in pigs is a common disease, causing severe diarrhea, that can result in substantial economic losses to the pig industry, but the molecular mechanisms and pathogenicity of this disease are not fully understood. The objective of this study was to identify the differentially expressed genes (DEGs) and long noncoding RNAs (DELs) related to inflammation in the colon tissues of pigs under constant (1, 7, and 14 days) HS.

Results: LncRNA and targeted gene interaction networks were constructed. GO annotation and KEGG pathway analyses were subsequently performed to determine the functions of the DEGs and DELs. The results revealed 57, 212, and 54 DEGs and 87, 79, and 55 DELs in the CON/H01, CON/H07, and CON/H14 groups, respectively. KRT85, CLDN1, S100A12, TM7SF2, CCN1, NR4A1, and several lncRNAs may be involved in regulating the development of intestinal inflammation. GO analysis indicated that the DEGs and DELs were enriched in a series of biological processes involved in the innate immune response, RAGE receptor binding, and positive regulation of the ERK1 and ERK2 cascades. KEGG pathways related to inflammation, such as the tight junction (TJ) and MAPK signaling pathways, were enriched in DEGs and DELs.

Conclusions: This study have expanded the knowledge about colon inflammation-related genes and lncRNA biology in pigs under HS; analyzed the the lncRNA‒mRNA interaction for HS-induced intestinal inflammation. These results may provide some references for our understanding of the molecular mechanism of the intestinal response to HS in pig.

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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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