Qingyuan Zheng, Ming Cui, Jinheng Xiao, Sen Yang, Tianqi Chen, Yanan Shi, Ya Hu, Quan Liao
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引用次数: 0
摘要
目的：甲状旁腺癌（PC）是一种罕见的恶性肿瘤，预后较差。在临床实践中，PC 的诊断通常比较困难，因此仍然需要有效的诊断标志物来进行鉴别诊断。凝集素芯片已在多种癌症中发现糖蛋白的糖基化异常，但在 PC 中却缺乏相关信息：方法：本研究使用由 70 种凝集素组成的芯片对 8 例 PC 和 6 例甲状旁腺腺瘤（PA）组织进行了评估。比较了PA和PC组织的整体凝集素特异性糖基化模式。通过凝集素组织化学法进一步验证了在PC和PA之间有明显差异反应的凝集素：结果：71.4%（50/70）的凝集素信号强度在两组间存在差异（P 1），其中马齿苋（ACL）在两组间的反应差异最大（比值比 = 2.45）。凝集素组织化学进一步证实，PC 中的 ACL 强度高于 PA。PC 组织中差异表达的聚糖主要以葡萄糖、甘露糖和半乳糖为主：PC呈现出独特的糖化学特征，ACL可作为PC的候选诊断标志物。
Glycomic profiling of parathyroid neoplasms via lectin microarray analysis.
Purpose: Parathyroid carcinoma (PC) is a rare malignancy with a poor prognosis. Diagnosis of PC is often difficult in clinical practice and efficient diagnostic markers are still needed for differential diagnosis. Aberrant glycosylations of glycoproteins were identified with lectin microarray in various cancers, while relevant information is lacking in PC.
Methods: In this study, 8 PC and 6 parathyroid adenoma (PA) tissues were assessed using a microarray consisting of 70 lectins. Overall lectin-specific glycosylation patterns were compared between PA and PC tissues. Lectins with significant differential response between PC and PA were further validated by lectin histochemistry.
Results: The difference in signal intensities was found in 71.4% (50/70) of the lectins between the two groups (P < 0.05). The vast majority of PCs had higher intensity signals than PAs (PCs vs. PAs, ratio >1) and amaranthus caudatus (ACL) showed the most significantly different response between them (ratio = 2.45). Lectin histochemistry further confirmed higher ACL intensity in PCs than in PAs. The differentially expressed glycans in PC tissues were primarily glucose, mannose, and galactose-based.
Conclusion: PC presented unique glycomic features and ACL may serve as a candidate diagnostic marker for PC.
期刊介绍：
Well-established as a major journal in today’s rapidly advancing experimental and clinical research areas, Endocrine publishes original articles devoted to basic (including molecular, cellular and physiological studies), translational and clinical research in all the different fields of endocrinology and metabolism. Articles will be accepted based on peer-reviews, priority, and editorial decision. Invited reviews, mini-reviews and viewpoints on relevant pathophysiological and clinical topics, as well as Editorials on articles appearing in the Journal, are published. Unsolicited Editorials will be evaluated by the editorial team. Outcomes of scientific meetings, as well as guidelines and position statements, may be submitted. The Journal also considers special feature articles in the field of endocrine genetics and epigenetics, as well as articles devoted to novel methods and techniques in endocrinology.
Endocrine covers controversial, clinical endocrine issues. Meta-analyses on endocrine and metabolic topics are also accepted. Descriptions of single clinical cases and/or small patients studies are not published unless of exceptional interest. However, reports of novel imaging studies and endocrine side effects in single patients may be considered. Research letters and letters to the editor related or unrelated to recently published articles can be submitted.
Endocrine covers leading topics in endocrinology such as neuroendocrinology, pituitary and hypothalamic peptides, thyroid physiological and clinical aspects, bone and mineral metabolism and osteoporosis, obesity, lipid and energy metabolism and food intake control, insulin, Type 1 and Type 2 diabetes, hormones of male and female reproduction, adrenal diseases pediatric and geriatric endocrinology, endocrine hypertension and endocrine oncology.
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