提出新的耐高温杆菌(Acinetobacter thermotolerans sp.nov.),以适应在 47 °C 下生长的牛粪便栖息细菌。

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Systematic and applied microbiology Pub Date : 2024-11-01 DOI:10.1016/j.syapm.2024.126560
Violetta Shestivska , Petra Španělová , Marcela Krůtová , Martina Maixnerová , Priscila Thiago Dobbler , Tomáš Větrovský , Alexandr Nemec , Martina Kyselková
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引用次数: 0

摘要

本研究旨在确定牛杆菌属新菌株的分类地位,这些菌株的特点是能在 44 °C以上的温度下生长,实属罕见。最初,根据初步的全细胞 MALDI-TOF MS 鉴定和 rpoB 基因测序,从 2022 年捷克 11 个农场收集的牛粪便中分离出 24 株菌株,代表了一个暂定的新物种。我们对包含组内多样性的 12 个菌株进行了详细研究,包括全基因组从头测序。基于核心基因组的系统发育分析表明,这 12 株菌株在该属中形成了一个独特的支系。该支系内基于 BLAST 的基因组平均核苷酸同一性(ANIb)值≥ 98.2%,而该支系与已知醋酸杆菌物种之间的 ANIb 值为 T(= CCM 9356T = CCUG 77195T = CNCTC 8200T)。
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Proposal of Acinetobacter thermotolerans sp. nov. to accommodate bovine feces-dwelling bacteria growing at 47 °C
This study aimed to determine the taxonomic status of a new group of bovine strains of the genus Acinetobacter characterized by the rare ability to grow at temperatures above 44 °C. Initially, 24 strains were isolated from cattle feces collected at 11 farms in Czechia in 2022, representing a tentative new species based on preliminary whole-cell MALDI-TOF MS identification and rpoB gene sequencing. Twelve strains encompassing the within-group diversity were studied in detail, including whole genome de novo sequencing. Core genome-based phylogenetic analysis revealed that the 12 strains form a distinct clade within the genus. The genomic average nucleotide identity based on BLAST (ANIb) values within the clade were ≥ 98.2%, whereas the ANIb values between the clade and the known Acinetobacter species were < 83%. Each of the 12 genomes consisted of a circular chromosome (3.05−3.28 Mb) and 1−6 extrachromosomal elements (1.43−122.5 kb). All strains were non-glucose oxidizing, non-hemolytic, non-proteolytic, and prototrophic, and used acetate, ethanol, and DL-lactate as sole carbon and energy sources. Unrestricted growth at 47 °C was their unique diagnostic characteristic in a genus-wide comparative analysis and their growth kinetics at 37–46 °C differed from that of Acinetobacter baumannii. The studied strains were susceptible to 18 antibiotics except for sporadic resistance to streptomycin, tetracycline, and sulfonamides associated with the presence of strA/strB, tet(Y), and sul2 genes, respectively. We conclude that the studied strains represent a new species, for which we propose the name Acinetobacter thermotolerans sp. nov. The type strain is ANC 7454T (= CCM 9356T = CCUG 77195T = CNCTC 8200T).
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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