{"title":"转谷氨酰胺酶 2 在特发性牙龈纤维瘤病中促进大聚糖合成的作用","authors":"Yurika Ninomiya, Shinji Matsuda, Shigeki Suzuki, Shizu Hirata-Tsuchiya, Tomoya Ueda, Fuminori Nakashima, Keisuke Yasuda, Shogo Shimada, Takumi Memida, Tetsuya Yoshimoto, Satoru Yamada, Kazuhisa Ouhara, Noriyoshi Mizuno","doi":"10.1186/s12903-024-05211-8","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).</p><p><strong>Methods: </strong>Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined.</p><p><strong>Results: </strong>RNA sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs.</p><p><strong>Conclusion: </strong>BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1422"},"PeriodicalIF":2.6000,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583685/pdf/","citationCount":"0","resultStr":"{\"title\":\"Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.\",\"authors\":\"Yurika Ninomiya, Shinji Matsuda, Shigeki Suzuki, Shizu Hirata-Tsuchiya, Tomoya Ueda, Fuminori Nakashima, Keisuke Yasuda, Shogo Shimada, Takumi Memida, Tetsuya Yoshimoto, Satoru Yamada, Kazuhisa Ouhara, Noriyoshi Mizuno\",\"doi\":\"10.1186/s12903-024-05211-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).</p><p><strong>Methods: </strong>Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined.</p><p><strong>Results: </strong>RNA sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs.</p><p><strong>Conclusion: </strong>BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF.</p>\",\"PeriodicalId\":9072,\"journal\":{\"name\":\"BMC Oral Health\",\"volume\":\"24 1\",\"pages\":\"1422\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-11-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583685/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Oral Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s12903-024-05211-8\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Oral Health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12903-024-05211-8","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.
Background: This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).
Methods: Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined.
Results: RNA sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs.
Conclusion: BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF.
期刊介绍：
BMC Oral Health is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of disorders of the mouth, teeth and gums, as well as related molecular genetics, pathophysiology, and epidemiology.
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