Abdullah Haikal , Mona El-Neketi , Manar G. Helal , Laila A. Abou-zeid , Madiha A. Hassan , Ahmed A. Gohar
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Gohar","doi":"10.1016/j.jep.2024.119133","DOIUrl":null,"url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Mentha longifolia</em> L. has been employed to treat cough, lung inflammation, and bronchial asthma disorders.</div></div><div><h3>Aim of the study</h3><div>Our study was carried out to investigate the medicinal effect of the flavonoids derived from <em>M. longifolia</em>, specifically didymin, linarin, rutin, and TMF, as well as the whole extracts of <em>M. longifolia</em> subsp. <em>typhoides</em> and <em>M. longifolia</em> subsp. <em>schimperi,</em> in comparison to dexa, in a mice model of ovalbumin-allergic asthma (OVA).</div></div><div><h3>Methods</h3><div>After inhaling OVA, the mice developed acute asthma symptoms. Mice were subjected orally to Dexa and/or isolated flavonoids. The study assessed total and differential leukocyte counts, LDH concentration, and total protein concentration in BALF, reduced levels of GSH and total NOx products in lung tissues and analyzed the lung specimens by staining them with hematoxylin and eosin (H & E).</div></div><div><h3>Results</h3><div>Histopathological analysis of the right lung lobes demonstrated that the isolated flavonoids exhibited a significant anti-inflammatory effect higher than Dexa as shown by decreasing the overall and distinct leukocyte counts, LDH levels, and total protein levels in BALF, as compared to the OVA group (p < 0.05). TMF was the most effective and the other tested flavonoids are more effective than Dexa but less than TMF. In addition, all tested flavonoids and Dexa significantly (p < 0.05) mitigated OVA-induced oxidative stress as evidenced by diminished lung NOx level and elevated GSH level. Computational docking studies proved recognition of didymin, linarin, rutin, and TMF to the human leukocyte elastase binding sites.</div></div><div><h3>Conclusion</h3><div>The tested flavonoids; didymin, linarin, rutin, and TMF successfully inhibit Ova-induced allergic asthma in mice through their anti-inflammatory and antioxidant activities and may represent promising candidate as a remedy for allergic asthma.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"339 ","pages":"Article 119133"},"PeriodicalIF":4.8000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti-asthmatic and antioxidant activity of flavonoids isolated from Mentha longifolia subspecies typhoides (Briq.) Harley. and Mentha longifolia subspecies schimperi (Briq.) 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Mice were subjected orally to Dexa and/or isolated flavonoids. The study assessed total and differential leukocyte counts, LDH concentration, and total protein concentration in BALF, reduced levels of GSH and total NOx products in lung tissues and analyzed the lung specimens by staining them with hematoxylin and eosin (H & E).</div></div><div><h3>Results</h3><div>Histopathological analysis of the right lung lobes demonstrated that the isolated flavonoids exhibited a significant anti-inflammatory effect higher than Dexa as shown by decreasing the overall and distinct leukocyte counts, LDH levels, and total protein levels in BALF, as compared to the OVA group (p < 0.05). TMF was the most effective and the other tested flavonoids are more effective than Dexa but less than TMF. In addition, all tested flavonoids and Dexa significantly (p < 0.05) mitigated OVA-induced oxidative stress as evidenced by diminished lung NOx level and elevated GSH level. 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引用次数: 0
摘要
民族药理学意义：Mentha longifolia L.被用于治疗咳嗽、肺部炎症和支气管哮喘疾病：我们的研究旨在探讨从龙胆草中提取的黄酮类化合物（特别是地丁、亚麻仁、芦丁和 TMF）以及龙胆草亚种和龙胆草亚种的全提取物与地塞米松相比，在卵清蛋白过敏性哮喘（OVA）小鼠模型中的药用效果：方法：小鼠吸入 OVA 后出现急性哮喘症状。小鼠口服地塞米松和/或分离的黄酮类化合物。研究评估了白细胞总数和差值、LDH 浓度、BALF 中的总蛋白浓度、肺组织中 GSH 和总氮氧化物产物的降低水平，并用血色素和伊红（H & E）染色分析了肺部标本：右肺叶的组织病理学分析表明，与 OVA 组相比，分离出的黄酮类化合物降低了 BALF 中的白细胞总数和不同白细胞计数、LDH 水平和总蛋白水平（p< 0.05），显示其显著的抗炎效果高于地塞米松（Dexa）。TMF的效果最好，其他测试的黄酮类化合物的效果高于Dexa，但低于TMF。此外，所有测试的黄酮类化合物和 Dexa 都能显著（p< 0.05）减轻 OVA 诱导的氧化应激，肺 NOx 水平降低和 GSH 水平升高就是证明。计算对接研究证明，地黄素、亚麻仁素、芦丁和TMF与白三烯酯酶结合位点相识别：结论：所测试的黄酮类化合物（地黄素、亚麻仁素、芦丁和 TMF）通过其抗炎和抗氧化活性成功地抑制了卵巢诱导的小鼠过敏性哮喘，可作为治疗过敏性哮喘的药物。
Anti-asthmatic and antioxidant activity of flavonoids isolated from Mentha longifolia subspecies typhoides (Briq.) Harley. and Mentha longifolia subspecies schimperi (Briq.) Briq. on ovalbumin-induced allergic asthma in mice: In-vivo and in-silico study
Ethnopharmacological relevance
Mentha longifolia L. has been employed to treat cough, lung inflammation, and bronchial asthma disorders.
Aim of the study
Our study was carried out to investigate the medicinal effect of the flavonoids derived from M. longifolia, specifically didymin, linarin, rutin, and TMF, as well as the whole extracts of M. longifolia subsp. typhoides and M. longifolia subsp. schimperi, in comparison to dexa, in a mice model of ovalbumin-allergic asthma (OVA).
Methods
After inhaling OVA, the mice developed acute asthma symptoms. Mice were subjected orally to Dexa and/or isolated flavonoids. The study assessed total and differential leukocyte counts, LDH concentration, and total protein concentration in BALF, reduced levels of GSH and total NOx products in lung tissues and analyzed the lung specimens by staining them with hematoxylin and eosin (H & E).
Results
Histopathological analysis of the right lung lobes demonstrated that the isolated flavonoids exhibited a significant anti-inflammatory effect higher than Dexa as shown by decreasing the overall and distinct leukocyte counts, LDH levels, and total protein levels in BALF, as compared to the OVA group (p < 0.05). TMF was the most effective and the other tested flavonoids are more effective than Dexa but less than TMF. In addition, all tested flavonoids and Dexa significantly (p < 0.05) mitigated OVA-induced oxidative stress as evidenced by diminished lung NOx level and elevated GSH level. Computational docking studies proved recognition of didymin, linarin, rutin, and TMF to the human leukocyte elastase binding sites.
Conclusion
The tested flavonoids; didymin, linarin, rutin, and TMF successfully inhibit Ova-induced allergic asthma in mice through their anti-inflammatory and antioxidant activities and may represent promising candidate as a remedy for allergic asthma.
期刊介绍：
The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.
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