{"title":"基于强度传输方程的光子计数三维荧光成像。","authors":"Shiori Matsuda, Marin Shoda, Naru Yoneda, Manoj Kumar, Takashi Murata, Inbarasan Muniraj, Yasuhiro Awatsuji, Osamu Matoba","doi":"10.1364/OE.540000","DOIUrl":null,"url":null,"abstract":"<p><p>A transport of intensity equation (TIE) based three-dimensional (3D) fluorescence imaging using photon-counting detection was proposed for low light-level bioimaging applications. The number of photons required to achieve the quantitative phase measurement using the proposed system was investigated experimentally and numerically. Results show that the feasibility of reconstructing phase information with an average number of photons is greater than about 9 per pixel in our plant cells.</p>","PeriodicalId":19691,"journal":{"name":"Optics express","volume":"32 23","pages":"42005-42015"},"PeriodicalIF":3.2000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Photon-counting three-dimensional fluorescence imaging based on the transport of intensity equation.\",\"authors\":\"Shiori Matsuda, Marin Shoda, Naru Yoneda, Manoj Kumar, Takashi Murata, Inbarasan Muniraj, Yasuhiro Awatsuji, Osamu Matoba\",\"doi\":\"10.1364/OE.540000\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A transport of intensity equation (TIE) based three-dimensional (3D) fluorescence imaging using photon-counting detection was proposed for low light-level bioimaging applications. The number of photons required to achieve the quantitative phase measurement using the proposed system was investigated experimentally and numerically. Results show that the feasibility of reconstructing phase information with an average number of photons is greater than about 9 per pixel in our plant cells.</p>\",\"PeriodicalId\":19691,\"journal\":{\"name\":\"Optics express\",\"volume\":\"32 23\",\"pages\":\"42005-42015\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-11-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Optics express\",\"FirstCategoryId\":\"101\",\"ListUrlMain\":\"https://doi.org/10.1364/OE.540000\",\"RegionNum\":2,\"RegionCategory\":\"物理与天体物理\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"OPTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optics express","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1364/OE.540000","RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPTICS","Score":null,"Total":0}
Photon-counting three-dimensional fluorescence imaging based on the transport of intensity equation.
A transport of intensity equation (TIE) based three-dimensional (3D) fluorescence imaging using photon-counting detection was proposed for low light-level bioimaging applications. The number of photons required to achieve the quantitative phase measurement using the proposed system was investigated experimentally and numerically. Results show that the feasibility of reconstructing phase information with an average number of photons is greater than about 9 per pixel in our plant cells.
期刊介绍：
Optics Express is the all-electronic, open access journal for optics providing rapid publication for peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.
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