Rong-xin Zhang, Yi Liu, Dian-nan Huang, Li Zhang, Xing-guan Ma, Peng-fei Yu, Chuang Liu, Yong-yong Wang
{"title":"油页岩蒸馏废水处理 MBBR 的进水负荷对硝化/反硝化的影响：性能和微生物群落结构","authors":"Rong-xin Zhang, Yi Liu, Dian-nan Huang, Li Zhang, Xing-guan Ma, Peng-fei Yu, Chuang Liu, Yong-yong Wang","doi":"10.1007/s11270-024-07615-2","DOIUrl":null,"url":null,"abstract":"<div><p>Oil shale retorting wastewater is currently recognized as one of the most complex industrial wastewater due to its ultra-low chemical oxygen demand to total nitrogen (C/N) ratio, high organic load, and complex chemical composition. In this paper, the effect of influent load on the performance and microbial community changes of moving bed biofilm reactor(MBBR) in the treatment of highly polluted retorting wastewater was investigated. The MBBR was proposed and demonstrated to be efficient in treating the retorting wastewater, containing COD and NH<sub>4</sub><sup>+</sup>-N of 5000-10000mg/L and 3000-5000mg/L. The removal efficiency of COD and NH<sub>4</sub><sup>+</sup>-N increased steadily in 1-79d, and the effect was the best when the influent load was 0.0242–0.0312kg COD/kg MLSS·d and 0.0173–0.0208kg NH<sub>4</sub><sup>+</sup>-N/kg MLSS·d. The removal efficiency of COD was up to 83.46%, and the removal efficiency of NH<sub>4</sub><sup>+</sup>-N was 82.08%. The long-term operation showed that the biofilm could be rapidly formed after 40 days. Scanning electron microscope observation showed that cocci and short bacilli appeared in the pores of the fillers. With the increase of influent load, the total amount of extracellular polymeric substances increased significantly, in which the content of protein increased sharply in stage III, while the polysaccharide was in a state of continuous and stable growth. High-throughput sequencing analysis showed that the nitrifying functional genus Nitrosomonas was highly enriched in the MBBR system (0 → 17.55%) and played a key role in the high accumulation of NO<sub>3</sub><sup>−</sup>-N. The newly added denitrifying bacteria <i>Brevundimonas</i> (5.85%) and <i>Reyranella</i> (4.57%) enhanced the degradation of COD and maintained the nitrogen cycle in the system. The enrichment of <i>Acinetobacter</i>, <i>Rugosibacter</i>, and <i>unclassified_Syntrophobacteraceae</i> in the system played an important role in the degradation of complex organic compounds such as polycyclic aromatic hydrocarbons and aromatic compounds.</p></div>","PeriodicalId":808,"journal":{"name":"Water, Air, & Soil Pollution","volume":"236 1","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Influence of Influent Load on Nitrification/Denitrification with MBBR for Oil Shale Retorting Wastewater Treatment: Performance and Microbial Community Structure\",\"authors\":\"Rong-xin Zhang, Yi Liu, Dian-nan Huang, Li Zhang, Xing-guan Ma, Peng-fei Yu, Chuang Liu, Yong-yong Wang\",\"doi\":\"10.1007/s11270-024-07615-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Oil shale retorting wastewater is currently recognized as one of the most complex industrial wastewater due to its ultra-low chemical oxygen demand to total nitrogen (C/N) ratio, high organic load, and complex chemical composition. In this paper, the effect of influent load on the performance and microbial community changes of moving bed biofilm reactor(MBBR) in the treatment of highly polluted retorting wastewater was investigated. The MBBR was proposed and demonstrated to be efficient in treating the retorting wastewater, containing COD and NH<sub>4</sub><sup>+</sup>-N of 5000-10000mg/L and 3000-5000mg/L. The removal efficiency of COD and NH<sub>4</sub><sup>+</sup>-N increased steadily in 1-79d, and the effect was the best when the influent load was 0.0242–0.0312kg COD/kg MLSS·d and 0.0173–0.0208kg NH<sub>4</sub><sup>+</sup>-N/kg MLSS·d. The removal efficiency of COD was up to 83.46%, and the removal efficiency of NH<sub>4</sub><sup>+</sup>-N was 82.08%. The long-term operation showed that the biofilm could be rapidly formed after 40 days. Scanning electron microscope observation showed that cocci and short bacilli appeared in the pores of the fillers. With the increase of influent load, the total amount of extracellular polymeric substances increased significantly, in which the content of protein increased sharply in stage III, while the polysaccharide was in a state of continuous and stable growth. High-throughput sequencing analysis showed that the nitrifying functional genus Nitrosomonas was highly enriched in the MBBR system (0 → 17.55%) and played a key role in the high accumulation of NO<sub>3</sub><sup>−</sup>-N. The newly added denitrifying bacteria <i>Brevundimonas</i> (5.85%) and <i>Reyranella</i> (4.57%) enhanced the degradation of COD and maintained the nitrogen cycle in the system. The enrichment of <i>Acinetobacter</i>, <i>Rugosibacter</i>, and <i>unclassified_Syntrophobacteraceae</i> in the system played an important role in the degradation of complex organic compounds such as polycyclic aromatic hydrocarbons and aromatic compounds.</p></div>\",\"PeriodicalId\":808,\"journal\":{\"name\":\"Water, Air, & Soil Pollution\",\"volume\":\"236 1\",\"pages\":\"\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-11-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Water, Air, & Soil Pollution\",\"FirstCategoryId\":\"6\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11270-024-07615-2\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water, Air, & Soil Pollution","FirstCategoryId":"6","ListUrlMain":"https://link.springer.com/article/10.1007/s11270-024-07615-2","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Influence of Influent Load on Nitrification/Denitrification with MBBR for Oil Shale Retorting Wastewater Treatment: Performance and Microbial Community Structure
Oil shale retorting wastewater is currently recognized as one of the most complex industrial wastewater due to its ultra-low chemical oxygen demand to total nitrogen (C/N) ratio, high organic load, and complex chemical composition. In this paper, the effect of influent load on the performance and microbial community changes of moving bed biofilm reactor(MBBR) in the treatment of highly polluted retorting wastewater was investigated. The MBBR was proposed and demonstrated to be efficient in treating the retorting wastewater, containing COD and NH4+-N of 5000-10000mg/L and 3000-5000mg/L. The removal efficiency of COD and NH4+-N increased steadily in 1-79d, and the effect was the best when the influent load was 0.0242–0.0312kg COD/kg MLSS·d and 0.0173–0.0208kg NH4+-N/kg MLSS·d. The removal efficiency of COD was up to 83.46%, and the removal efficiency of NH4+-N was 82.08%. The long-term operation showed that the biofilm could be rapidly formed after 40 days. Scanning electron microscope observation showed that cocci and short bacilli appeared in the pores of the fillers. With the increase of influent load, the total amount of extracellular polymeric substances increased significantly, in which the content of protein increased sharply in stage III, while the polysaccharide was in a state of continuous and stable growth. High-throughput sequencing analysis showed that the nitrifying functional genus Nitrosomonas was highly enriched in the MBBR system (0 → 17.55%) and played a key role in the high accumulation of NO3−-N. The newly added denitrifying bacteria Brevundimonas (5.85%) and Reyranella (4.57%) enhanced the degradation of COD and maintained the nitrogen cycle in the system. The enrichment of Acinetobacter, Rugosibacter, and unclassified_Syntrophobacteraceae in the system played an important role in the degradation of complex organic compounds such as polycyclic aromatic hydrocarbons and aromatic compounds.
期刊介绍：
Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments.
Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation.
Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
