Simultaneous Quantitation of Bioactive Compounds in Different Varieties of Arachis Hypogea L. Using Liquid Chromatography/ESI Mass Spectrometry: A Chemometric Approach

Cultivated Arachis hypogaea L., is commonly known as peanut. Its seed coat, also referred to as a shell, plays a critical role in safeguarding the seeds and facilitating the transportation of diverse metabolites. In the current work, an examination of metabolite profiling was executed utilizing UPLC-ESI–MS/MS in the seed coat (SeC), seed skin (SeS), and seed pulp (SeP) of four distinct peanut varieties of different geographical locations in India. The method was also validated according to ICH guidelines. A linear detector response was observed within a concentration range of 0.019–20.0 ng/mL, R² = 0.999. The limit of detection (LOD) and quantification (LOQ) of the targeted compounds were found to be within the range of 0.075–0.426 and 0.227–1.292 ng/mL, respectively. The intra-day and inter-day precision demonstrated relative standard deviation %RSD < 2%. The results of Principal Component Analysis (PCA) have demonstrated that the differentiation between varieties of peanut is attributable to the existence of luteolin and rutin having a greater impact on the identification of varieties.

Graphical Abstract