{"title":"丁后马钱子（Maytenus emarginata (Willd.) Ding Hou）中羽扇豆醇（Lupeol）、β-香树脂醇（β-Amyrin）和豆甾醇（Stigmasterol）的 RP-HPLC 同时定量测定方法的验证","authors":"Supriya Sharma, Mahaveer Dhobi","doi":"10.1007/s10337-024-04365-5","DOIUrl":null,"url":null,"abstract":"<div><p>The study aims at developing and validating a RP-HPLC (reverse phase high-performance liquid chromatography) method for the standardization of <i>Maytenus emarginata</i> (Willd.) Ding Hou extract by selecting Lupeol, β-Amyrin, and Stigmasterol as marker compounds. The developed analytical method used a Phenomenex C<sub>8</sub> column (250 × 4.6 mm; 5 μm) and the mobile phase of acetonitrile and HPLC (high-performance liquid chromatography) grade water (85:15) in isocratic elution at the flow rate 2.0 ml/min. Further, the compounds were traced at 202 nm with the run time of 9 min. The calibration curves presented good linear regression (<i>R</i><sup>2</sup> > 0.999) in the tested concentrations. The developed method represented good repeatability for the estimation of the three compounds, namely Lupeol (0.009%), β-Amyrin (0.010%), and Stigmasterol (0.011%) in the samples having the RSD (relative standard deviation) of < 2% respectively. For all analytes, percentage recovery surpassed 90%. The validated RP-HPLC Method was utilized to quantify Lupeol, β-Amyrin, and Stigmasterol from methanolic extract of aerial parts of <i>Maytenus emarginata</i> (Willd.) Ding Hou.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 11-12","pages":"709 - 719"},"PeriodicalIF":1.2000,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Validated RP-HPLC Method for Simultaneous Quantitative Determination of Lupeol, β-Amyrin, and Stigmasterol in Maytenus emarginata (Willd.) Ding Hou\",\"authors\":\"Supriya Sharma, Mahaveer Dhobi\",\"doi\":\"10.1007/s10337-024-04365-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The study aims at developing and validating a RP-HPLC (reverse phase high-performance liquid chromatography) method for the standardization of <i>Maytenus emarginata</i> (Willd.) Ding Hou extract by selecting Lupeol, β-Amyrin, and Stigmasterol as marker compounds. The developed analytical method used a Phenomenex C<sub>8</sub> column (250 × 4.6 mm; 5 μm) and the mobile phase of acetonitrile and HPLC (high-performance liquid chromatography) grade water (85:15) in isocratic elution at the flow rate 2.0 ml/min. Further, the compounds were traced at 202 nm with the run time of 9 min. The calibration curves presented good linear regression (<i>R</i><sup>2</sup> > 0.999) in the tested concentrations. The developed method represented good repeatability for the estimation of the three compounds, namely Lupeol (0.009%), β-Amyrin (0.010%), and Stigmasterol (0.011%) in the samples having the RSD (relative standard deviation) of < 2% respectively. For all analytes, percentage recovery surpassed 90%. The validated RP-HPLC Method was utilized to quantify Lupeol, β-Amyrin, and Stigmasterol from methanolic extract of aerial parts of <i>Maytenus emarginata</i> (Willd.) Ding Hou.</p></div>\",\"PeriodicalId\":518,\"journal\":{\"name\":\"Chromatographia\",\"volume\":\"87 11-12\",\"pages\":\"709 - 719\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2024-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromatographia\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10337-024-04365-5\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromatographia","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s10337-024-04365-5","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
A Validated RP-HPLC Method for Simultaneous Quantitative Determination of Lupeol, β-Amyrin, and Stigmasterol in Maytenus emarginata (Willd.) Ding Hou
The study aims at developing and validating a RP-HPLC (reverse phase high-performance liquid chromatography) method for the standardization of Maytenus emarginata (Willd.) Ding Hou extract by selecting Lupeol, β-Amyrin, and Stigmasterol as marker compounds. The developed analytical method used a Phenomenex C8 column (250 × 4.6 mm; 5 μm) and the mobile phase of acetonitrile and HPLC (high-performance liquid chromatography) grade water (85:15) in isocratic elution at the flow rate 2.0 ml/min. Further, the compounds were traced at 202 nm with the run time of 9 min. The calibration curves presented good linear regression (R2 > 0.999) in the tested concentrations. The developed method represented good repeatability for the estimation of the three compounds, namely Lupeol (0.009%), β-Amyrin (0.010%), and Stigmasterol (0.011%) in the samples having the RSD (relative standard deviation) of < 2% respectively. For all analytes, percentage recovery surpassed 90%. The validated RP-HPLC Method was utilized to quantify Lupeol, β-Amyrin, and Stigmasterol from methanolic extract of aerial parts of Maytenus emarginata (Willd.) Ding Hou.
期刊介绍：
Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.
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