建立首个永生化声带白斑上皮细胞系并确定其特征。

IF 4.8 3区医学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Cancer gene therapy Pub Date : 2024-11-23 DOI:10.1038/s41417-024-00859-4

Zi-Ming Fu, Yang-Yang Bao, Zhe Chen, Jiang-Tao Zhong, Heng-Chao Chen, Zai-Zai Cao, Shui-Hong Zhou

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引用次数: 0

摘要

声带白斑（VCL）在喉癌的病因和发展过程中的重要性已得到越来越多的认可。然而，由于声带白斑病上皮细胞体积小、培养寿命有限，对声带白斑病等喉癌前病变机制的研究一直受到阻碍。在本研究中，我们改进了VCL上皮细胞的原代培养方案，并引入了模拟病毒40大T，建立了永生化细胞系，命名为hVCL-MSDEP01。我们证实，hVCL-MSDEP01 能表达上皮特异性基因和蛋白；与原代细胞相比，它还表现出独特的细胞周期动力学和凋亡率。总之，hVCL-MSDEP01 是研究 VCL 的理想体外模型。该细胞系将极大地推动喉癌病因和进展的研究。

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Establishment and characterization of the first immortalized vocal cord leukoplakia epithelial cell line.

The importance of vocal cord leukoplakia (VCL) in the etiology and progression of laryngeal carcinoma has gained increasing recognition. However, research into the mechanisms of laryngeal precancerous lesions such as VCL, has been hampered by the small size of VCL epithelial cells and their limited culture lifespan. In this study, we enhanced the primary culture protocol for VCL epithelial cells and introduced simian virus 40 Large T to establish an immortalized cell line, designated hVCL-MSDEP01. We confirmed that hVCL-MSDEP01 expresses epithelial-specific genes and proteins; it also demonstrates distinct cell cycle dynamics and apoptosis rates compared with primary cells. In conclusion, hVCL-MSDEP01 serves as an ideal in vitro model for studying VCL. This cell line will substantially advance research into the etiology and progression of laryngeal carcinoma.

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来源期刊

Cancer gene therapy 医学-生物工程与应用微生物

CiteScore

10.20

自引率

0.00%

发文量

150

审稿时长

4-8 weeks

期刊介绍： Cancer Gene Therapy is the essential gene and cellular therapy resource for cancer researchers and clinicians, keeping readers up to date with the latest developments in gene and cellular therapies for cancer. The journal publishes original laboratory and clinical research papers, case reports and review articles. Publication topics include RNAi approaches, drug resistance, hematopoietic progenitor cell gene transfer, cancer stem cells, cellular therapies, homologous recombination, ribozyme technology, antisense technology, tumor immunotherapy and tumor suppressors, translational research, cancer therapy, gene delivery systems (viral and non-viral), anti-gene therapy (antisense, siRNA & ribozymes), apoptosis; mechanisms and therapies, vaccine development, immunology and immunotherapy, DNA synthesis and repair. Cancer Gene Therapy publishes the results of laboratory investigations, preclinical studies, and clinical trials in the field of gene transfer/gene therapy and cellular therapies as applied to cancer research. Types of articles published include original research articles; case reports; brief communications; review articles in the main fields of drug resistance/sensitivity, gene therapy, cellular therapy, tumor suppressor and anti-oncogene therapy, cytokine/tumor immunotherapy, etc.; industry perspectives; and letters to the editor.