单、二价离子及其强度对婴儿配方蛋白质模型体系发泡特性的影响

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED Food Hydrocolloids Pub Date : 2024-11-08 DOI:10.1016/j.foodhyd.2024.110828

Siyu Zhang , Qinggang Xie , Fei Wang , Yaxing Xie , Jianjun Cheng , Qingfeng Ban

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引用次数: 0

摘要

金属离子可能会影响含有牛奶蛋白的婴儿配方奶粉在重组过程中的发泡特性。本研究构建了一个由乳清蛋白和酪蛋白组成的婴儿配方蛋白模型系统（IFPMS），并研究了一价（Na+、K+）和二价（Mg2+、Ca2+）离子及其浓度（0-100 mM）对发泡特性的影响。单离子对发泡能力（FC）没有明显影响，MgCl2 稍微增加了 FC，在 25 mM 时达到最大值 116.09 ± 7.46%，而 CaCl2 则显著降低了 FC，在 0-25 mM 时从 105.78 ± 7.42% 降至 83.14 ± 6.75%（p <0.05）。泡沫稳定性（FS）在所有 10 mM 以上的盐中都有明显改善（p < 0.05）。二价离子使 IFPMS 的净电荷急剧下降，蛋白质聚集体增大（25 mM），这表明二价离子具有更强的电荷屏蔽效应，从而导致蛋白质吸附缓慢，表面张力增加。多重光谱结果证实，盐类通过分子间作用增强了蛋白质的相互作用，并形成粘弹性层以稳定泡沫。吸收的蛋白质和 SDS-PAGE 分析表明，MgCl2 促进了蛋白质的吸附，而 CaCl2 则减少了泡沫相中的酪蛋白。相关性分析进一步表明，FC与吸收的蛋白质、溶解度和β-匝数呈极显著正相关（p ≤ 0.01），FS与二级结构和粘度呈显著相关（p ≤ 0.05）。这项研究可为深入了解婴儿配方奶粉的发泡特性提供有用信息。

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Effects of mono- and divalent-ions and their strength on foaming properties of infant formula protein model system

Metal ions may affect the foaming properties of infant formula products that including milk protein during reconstitution. In this study, an infant formula protein model system (IFPMS) composed of both whey protein and casein was constructed, and the effect of mono (Na⁺, K⁺) and divalent (Mg²⁺, Ca²⁺) ions and their strength (0–100 mM) on foaming properties was investigated. The mono ions had no significantly impact on foaming capacity (FC), MgCl₂ slightly increase FC and reaching a maximum of 116.09 ± 7.46% at 25 mM, whereas CaCl₂ significantly decreased FC from 105.78 ± 7.42% to 83.14 ± 6.75% as 0–25 mM (p < 0.05). Foam stability (FS) was significantly improved as all salts above 10 mM (p < 0.05). The dramatically decreased net charge and larger protein aggregates (>25 mM) of IFPMS with divalent ions indicating their stronger charge-shielding effect, which caused slow adsorption of protein and increased surface tension. Multiple spectral results confirmed that salts enhanced protein interactions via inter-molecular and form a viscoelastic layer to stabilize foam. Absorbed protein and SDS-PAGE analysis revealed that MgCl₂ promoted protein adsorption, while CaCl₂ reduced casein in the foam phase. Correlation analysis further implied that the FC showed highly significant positive with absorbed protein, solubility, and β-turn (p ≤ 0.01), and FS was significantly correlated with secondary structure and viscosity (p ≤ 0.05). This study may provide useful information for an in-depth understanding of the foaming properties of infant formula.

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.