{"title":"使用蓝光的 TONS504 光动力抗菌化疗对铜绿假单胞菌的体外疗效","authors":"Tomoya Furujo, Norihiko Ito, Kazuki Harada, Isao Sakata, Tomohiro Osaki","doi":"10.33160/yam.2024.11.001","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Pseudomonas aeruginosa</i> is an important causative agent of bacterial keratitis. This study investigates the antibacterial effect of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS504 (TONS504-PACT) and a blue light-emitting diode (LED) on <i>P. aeruginosa</i> <i>in vitro</i>. It also explores the synergistic effects of combining TONS504-PACT with EDTA, and compares the antibacterial effects of TONS504-PACT using blue light with those of previously reported red light.</p><p><strong>Methods: </strong>To evaluate the antibacterial effects of TONS504-PACT using blue light on <i>P. aeruginosa</i>, PACT with TONS504 (0-100 mg/L) was applied using a 405 nm LED at 0-30 J/cm<sup>2</sup> to <i>P. aeruginosa</i> (10<sup>7-8</sup> CFU/mL). The antibacterial effects were assessed by calculating the survival fraction. Subsequently, to compare the effects of TONS504-PACT using blue light with those of using red light, PACT with TONS504 (10 or 100 mg/L) was conducted using a 405 or 660 nm LED at 30 J/cm<sup>2</sup> and evaluated in the same manner. Finally, to investigate the synergistic antibacterial effects of TONS504-PACT with EDTA, PACT with TONS504 (0-100 mg/L) was applied using a 405 nm LED at 5 J/cm<sup>2</sup>, both with and without EDTA (0.015 M), and its antibacterial effects were similarly assessed.</p><p><strong>Results: </strong>TONS504-PACT using blue light decreased the survival of <i>P. aeruginosa</i>, depending on the light intensity. The greatest antibacterial effect was observed at 10 mg/L and 30 J/cm<sup>2</sup>, showing a survival fraction of 3.5 × 10<sup>-5</sup>. TONS504-PACT demonstrated a significantly greater antibacterial effect using blue light than that demonstrated using red light at 10 mg/L, though not at 100 mg/L. The combination of TONS504-PACT using blue light with EDTA significantly enhanced antibacterial effects at all concentrations.</p><p><strong>Conclusion: </strong>These findings confirm the effectiveness of TONS504-PACT using blue light against <i>P. aeruginosa</i> and suggest its potential as a treatment for bacterial keratitis.</p>","PeriodicalId":23795,"journal":{"name":"Yonago acta medica","volume":"67 4","pages":"284-292"},"PeriodicalIF":0.9000,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584238/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>In Vitro</i> Efficacy of Photodynamic Antimicrobial Chemotherapy with TONS504 Using Blue Light Against <i>Pseudomonas aeruginosa</i>.\",\"authors\":\"Tomoya Furujo, Norihiko Ito, Kazuki Harada, Isao Sakata, Tomohiro Osaki\",\"doi\":\"10.33160/yam.2024.11.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Pseudomonas aeruginosa</i> is an important causative agent of bacterial keratitis. This study investigates the antibacterial effect of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS504 (TONS504-PACT) and a blue light-emitting diode (LED) on <i>P. aeruginosa</i> <i>in vitro</i>. It also explores the synergistic effects of combining TONS504-PACT with EDTA, and compares the antibacterial effects of TONS504-PACT using blue light with those of previously reported red light.</p><p><strong>Methods: </strong>To evaluate the antibacterial effects of TONS504-PACT using blue light on <i>P. aeruginosa</i>, PACT with TONS504 (0-100 mg/L) was applied using a 405 nm LED at 0-30 J/cm<sup>2</sup> to <i>P. aeruginosa</i> (10<sup>7-8</sup> CFU/mL). The antibacterial effects were assessed by calculating the survival fraction. Subsequently, to compare the effects of TONS504-PACT using blue light with those of using red light, PACT with TONS504 (10 or 100 mg/L) was conducted using a 405 or 660 nm LED at 30 J/cm<sup>2</sup> and evaluated in the same manner. Finally, to investigate the synergistic antibacterial effects of TONS504-PACT with EDTA, PACT with TONS504 (0-100 mg/L) was applied using a 405 nm LED at 5 J/cm<sup>2</sup>, both with and without EDTA (0.015 M), and its antibacterial effects were similarly assessed.</p><p><strong>Results: </strong>TONS504-PACT using blue light decreased the survival of <i>P. aeruginosa</i>, depending on the light intensity. The greatest antibacterial effect was observed at 10 mg/L and 30 J/cm<sup>2</sup>, showing a survival fraction of 3.5 × 10<sup>-5</sup>. TONS504-PACT demonstrated a significantly greater antibacterial effect using blue light than that demonstrated using red light at 10 mg/L, though not at 100 mg/L. The combination of TONS504-PACT using blue light with EDTA significantly enhanced antibacterial effects at all concentrations.</p><p><strong>Conclusion: </strong>These findings confirm the effectiveness of TONS504-PACT using blue light against <i>P. aeruginosa</i> and suggest its potential as a treatment for bacterial keratitis.</p>\",\"PeriodicalId\":23795,\"journal\":{\"name\":\"Yonago acta medica\",\"volume\":\"67 4\",\"pages\":\"284-292\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584238/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Yonago acta medica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.33160/yam.2024.11.001\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/11/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Yonago acta medica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.33160/yam.2024.11.001","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
In Vitro Efficacy of Photodynamic Antimicrobial Chemotherapy with TONS504 Using Blue Light Against Pseudomonas aeruginosa.
Background: Pseudomonas aeruginosa is an important causative agent of bacterial keratitis. This study investigates the antibacterial effect of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS504 (TONS504-PACT) and a blue light-emitting diode (LED) on P. aeruginosain vitro. It also explores the synergistic effects of combining TONS504-PACT with EDTA, and compares the antibacterial effects of TONS504-PACT using blue light with those of previously reported red light.
Methods: To evaluate the antibacterial effects of TONS504-PACT using blue light on P. aeruginosa, PACT with TONS504 (0-100 mg/L) was applied using a 405 nm LED at 0-30 J/cm2 to P. aeruginosa (107-8 CFU/mL). The antibacterial effects were assessed by calculating the survival fraction. Subsequently, to compare the effects of TONS504-PACT using blue light with those of using red light, PACT with TONS504 (10 or 100 mg/L) was conducted using a 405 or 660 nm LED at 30 J/cm2 and evaluated in the same manner. Finally, to investigate the synergistic antibacterial effects of TONS504-PACT with EDTA, PACT with TONS504 (0-100 mg/L) was applied using a 405 nm LED at 5 J/cm2, both with and without EDTA (0.015 M), and its antibacterial effects were similarly assessed.
Results: TONS504-PACT using blue light decreased the survival of P. aeruginosa, depending on the light intensity. The greatest antibacterial effect was observed at 10 mg/L and 30 J/cm2, showing a survival fraction of 3.5 × 10-5. TONS504-PACT demonstrated a significantly greater antibacterial effect using blue light than that demonstrated using red light at 10 mg/L, though not at 100 mg/L. The combination of TONS504-PACT using blue light with EDTA significantly enhanced antibacterial effects at all concentrations.
Conclusion: These findings confirm the effectiveness of TONS504-PACT using blue light against P. aeruginosa and suggest its potential as a treatment for bacterial keratitis.
期刊介绍：
Yonago Acta Medica (YAM) is an electronic journal specializing in medical sciences, published by Tottori University Medical Press, 86 Nishi-cho, Yonago 683-8503, Japan.
The subject areas cover the following: molecular/cell biology; biochemistry; basic medicine; clinical medicine; veterinary medicine; clinical nutrition and food sciences; medical engineering; nursing sciences; laboratory medicine; clinical psychology; medical education.
Basically, contributors are limited to members of Tottori University and Tottori University Hospital. Researchers outside the above-mentioned university community may also submit papers on the recommendation of a professor, an associate professor, or a junior associate professor at this university community.
Articles are classified into four categories: review articles, original articles, patient reports, and short communications.
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