{"title":"基于 MXene 双金属涂层的增效增强型比色-拉曼双信号免疫层析分析仪，用于提高检测性能","authors":"Youwen Deng, Zhiheng Gao, Zhongqi Lin, Zehao Yang, Mengfang Lin, Zhenlin Xu, Hongtao Lei, Xiangmei Li","doi":"10.1021/acs.analchem.4c04234","DOIUrl":null,"url":null,"abstract":"Herein, a three-dimensional thin film-like multifunctional MXene bimetallic coating material (Ti<sub>3</sub>C<sub>2</sub>@Au–Ag) with strong color intensity, high surface-enhanced Raman scattering (SERS) activity, and strong antibody affinity (1.00 × 10<sup>8</sup> M<sup>–1</sup>) was prepared. It was the first time that Ti<sub>3</sub>C<sub>2</sub>@Au–Ag-based colorimetric-SERS dual-signal immunochromatographic assay (ICA) was developed for the detection of dexamethasone, achieving the limits of detection of 0.0089, 0.14, and 0.084 μg/kg for milk, beef, and pork in colorimetric mode and 0.0015, 0.060, and 0.075 μg/kg in SERS mode. It was up to 200-fold more sensitive than the reported ICAs. The recoveries were 82.0%–112.6%, and the coefficients of variation were 1.4%–13.7%. The Ti<sub>3</sub>C<sub>2</sub>@Au–Ag-ICA was verified by LC–MS/MS in the application on 30 real samples with a correlation coefficient greater than 0.98. This study can provide efficient theoretical and practical value for the development of a colorimetric-SERS dual-signal immunoassay platform.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"191 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"MXene Bimetallic Coating Synergistic Enhanced Colorimetric-Raman Dual Signal-Based Immunochromatographic Assay for Advancing Detection Performance\",\"authors\":\"Youwen Deng, Zhiheng Gao, Zhongqi Lin, Zehao Yang, Mengfang Lin, Zhenlin Xu, Hongtao Lei, Xiangmei Li\",\"doi\":\"10.1021/acs.analchem.4c04234\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Herein, a three-dimensional thin film-like multifunctional MXene bimetallic coating material (Ti<sub>3</sub>C<sub>2</sub>@Au–Ag) with strong color intensity, high surface-enhanced Raman scattering (SERS) activity, and strong antibody affinity (1.00 × 10<sup>8</sup> M<sup>–1</sup>) was prepared. It was the first time that Ti<sub>3</sub>C<sub>2</sub>@Au–Ag-based colorimetric-SERS dual-signal immunochromatographic assay (ICA) was developed for the detection of dexamethasone, achieving the limits of detection of 0.0089, 0.14, and 0.084 μg/kg for milk, beef, and pork in colorimetric mode and 0.0015, 0.060, and 0.075 μg/kg in SERS mode. It was up to 200-fold more sensitive than the reported ICAs. The recoveries were 82.0%–112.6%, and the coefficients of variation were 1.4%–13.7%. The Ti<sub>3</sub>C<sub>2</sub>@Au–Ag-ICA was verified by LC–MS/MS in the application on 30 real samples with a correlation coefficient greater than 0.98. This study can provide efficient theoretical and practical value for the development of a colorimetric-SERS dual-signal immunoassay platform.\",\"PeriodicalId\":27,\"journal\":{\"name\":\"Analytical Chemistry\",\"volume\":\"191 1\",\"pages\":\"\"},\"PeriodicalIF\":6.7000,\"publicationDate\":\"2024-11-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.analchem.4c04234\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.4c04234","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Herein, a three-dimensional thin film-like multifunctional MXene bimetallic coating material (Ti3C2@Au–Ag) with strong color intensity, high surface-enhanced Raman scattering (SERS) activity, and strong antibody affinity (1.00 × 108 M–1) was prepared. It was the first time that Ti3C2@Au–Ag-based colorimetric-SERS dual-signal immunochromatographic assay (ICA) was developed for the detection of dexamethasone, achieving the limits of detection of 0.0089, 0.14, and 0.084 μg/kg for milk, beef, and pork in colorimetric mode and 0.0015, 0.060, and 0.075 μg/kg in SERS mode. It was up to 200-fold more sensitive than the reported ICAs. The recoveries were 82.0%–112.6%, and the coefficients of variation were 1.4%–13.7%. The Ti3C2@Au–Ag-ICA was verified by LC–MS/MS in the application on 30 real samples with a correlation coefficient greater than 0.98. This study can provide efficient theoretical and practical value for the development of a colorimetric-SERS dual-signal immunoassay platform.
期刊介绍：
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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