Characterization of Multipurpose Isolate Pseudomonas aeruginosa AMBCD-1 for Biocontrol and Biodegradation Efficiency

The goal of the study was to assess the capability of the bacteria Pseudomonas aeruginosa AMBCD-1 for paddy straw degradation as well as its biological control abilities against the plant wilt pathogen Fusarium. Fourier-transform infrared spectroscopy (FTIR) data showed that AMBCD-1 culture-inoculated treatments dramatically reduced the levels of cellulose, lignin, and hemicelluloses in the deteriorated paddy straw. Additionally, a dual culture technique was utilized to investigate antagonistic activity in vitro using nutritional agar media and potato dextrose agar in equal proportions (1:1) for suppressing the growth of the fungus Fusarium oxysporum and Sclerotium rolfsii in order to assess the biocontrol properties of the chosen bacteria. Following incubation, it was shown that AMBCD-1 exhibited a 66.67% antibacterial activity against the fungus Fusarium oxysporum. Furthermore, the pyocyanin pigment was also extracted from the P. aeruginosa AMBCD-1 strain and confirmed at a mass value of 211.2 using liquid chromatography mass spectrometry (LC-MS), with its standard peak observed at 1.48 min. The results revealed that bacterium P. aeruginosa AMBCD-1 had the ability to biodegrade paddy straw and also inhibit the appearance of the wilt disease caused by Fusarium species in numerous crops.