从马来西亚肉鸡及其养殖场环境中分离出的产 ESBL 大肠杆菌的流行率和分子特征。

IF 4 2区生物学 Q2 MICROBIOLOGY BMC Microbiology Pub Date : 2024-11-26 DOI:10.1186/s12866-024-03653-2

Mulu Lemlem, Erkihun Aklilu, Maizan Mohamed, Nor Fadhilah Kamaruzzaman, Susmita Seenu Devan, Habiba Lawal, Abubakar Abdulkarim Kanamma

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引用次数: 0

摘要

背景：产广谱β-内酰胺酶大肠埃希菌（ESBL-EC）对公共健康的威胁日益严重。本研究旨在确定马来西亚吉兰丹州肉鸡及其养殖环境中分离出的产 ESBL 大肠杆菌（大肠杆菌）的流行率和特征：方法：从采集的 453 份样本（包括 210 份泄殖腔拭子和 243 份环境样本）中分离出大肠埃希氏菌。采用碟片扩散法评估了大肠杆菌分离物对 16 种抗生素的抗菌药敏感性。使用改良双盘协同法评估了大肠杆菌分离物产生 ESBL 的表型。提取基因组 DNA 后，使用适当的引物通过 PCR 检测 ESBL 耐药基因、系统发生群和毒力基因。通过测序进一步确认了 ESBL 基因。大肠杆菌菌株的分子分型是通过多焦点序列分型法（MLST）确定的：结果：从采集的样本中总共分离出 93.8%（425/453）的大肠杆菌。在筛选出的 334 个大肠杆菌分离物中，14.7%（49/334）表型为 ESBL 生产者。所有 ESBL-EC 均对四环素、环丙沙星和氨苄西林具有抗药性。因此，100%的ESBL-EC都具有多重耐药性。81.6%的ESBL-EC至少有一种ESBL编码基因呈阳性。检测到的最常见的 ESBL 基因是 blaTEM（77.6%；38/49），其次是 blaCTX-M（32.7%；16/49）和 blaSHV（18.4%；9/49）。大多数 ESBL-EC 属于系统发生群 A，其次是 B1，分别占 44.9% 和 12.2%。最常发现的序列类型是 ST10（3 个）和 ST206（3 个）。在大肠杆菌分离物中检测到最多的毒力基因是 astA（33.3%；22/66），其次是 iss（15.2%；10/66）：我们的研究结果表明，肉鸡及其养殖场环境都是产生多重耐药 ESBL 大肠杆菌和 ESBL 耐药基因的温床。

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Prevalence and molecular characterization of ESBL-producing Escherichia coli isolated from broiler chicken and their respective farms environment in Malaysia.

Background: Extended spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) is an increasing public health threat. This study aimed to determine the prevalence and characterization of ESBL-producing Escherichia coli (E. coli) isolated from broiler chicken and their farm environment, in Kelantan Malaysia.

Methods: Escherichia coli was isolated from 453 collected samples, including 210 cloacal swabs and 243 environmental samples. The antimicrobial susceptibility profile of the E. coli isolates was assessed for sixteen antibiotics using the disc diffusion method. The E. coli isolates were evaluated for phenotypic ESBL production using modified double disc synergy. After extraction of genomic DNA, ESBL resistance genes, phylogenetic group, and virulence genes were detected by PCR using appropriate primers. ESBL genes were further confirmed by sequencing. The molecular typing of E. coli strains was determined by Multilocus Sequence Typing (MLST).

Results: A total of 93.8% (425/453) E. coli were isolated from the collected samples. Out of 334 E. coli isolates screened, 14.7% (49/334) were phenotypically ESBL producers. All the ESBL-EC were resistant to tetracycline, ciprofloxacin, and ampicillin. Thus, 100% of the ESBL-EC were multidrug resistant. Of the ESBL-EC 81.6% were positive for at least one ESBL encoding gene. The most prevalent ESBL gene detected was bla_TEM (77.6%; 38/49) followed by bla_CTX-M (32.7%; 16/49) and bla_SHV (18.4%; 9/49). The majority of ESBL-EC belonged to phylogenic groups A followed by B1 accounting for 44.9% and 12.2%, respectively. The most frequently identified sequence types were ST10 (n = 3) and ST206 (n = 3). The most detected virulence genes in the E. coli isolates were astA (33.3%; 22/66) followed by iss (15.2%; 10/66).

Conclusions: Our results show both broiler chicken and their respective farms environment were reservoirs of multi-drug resistant ESBL-producing E. coli and ESBL resistance genes.

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来源期刊

BMC Microbiology 生物-微生物学

CiteScore

7.20

自引率

0.00%

发文量

280

审稿时长

3 months

期刊介绍： BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.