海洋红树林衍生真菌代谢物中环甘氨酸-脯氨酸的定量监测。

IF 4.2 2区生物学 Q2 MICROBIOLOGY Journal of Fungi Pub Date : 2024-11-10 DOI:10.3390/jof10110779

Jing Lin, Fei Qin, Zeye Lin, Weijian Lin, Minxin You, Li Xu, Lei Hu, Yung-Husan Chen

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引用次数: 0

摘要

本研究开发并验证了一种稳健的 UPLC-MS/MS 方法，用于定量检测红树林青霉和曲霉菌株中的环甘氨酸脯氨酸（cGP）。该方法具有良好的线性、精确度和回收率，检测限低至 4.8 纳克/毫升。赤拟青霉提取物的 cGP 含量为 67.45 ± 1.11 ng/mL，相应的发酵产率为 29.31 ± 0.61 mg/L。这超过了steckii青霉菌，后者的cGP含量为31.71 ± 0.31 ng/mL，产量为8.51 ± 0.15 mg/L。这种代谢物定量分析方法为筛选这些真菌菌株提供了一种可行的方法，凸显了它们可持续生产环甘氨酸脯氨酸（cGP）的潜力。

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Quantitative Monitoring of Cyclic Glycine-Proline in Marine Mangrove-Derived Fungal Metabolites.

This study developed and validated a robust UPLC-MS/MS method for quantifying cyclic glycine-proline (cGP) in mangrove-derived Penicillium and Aspergillus strains. The method demonstrated excellent linearity, precision, and recovery, with detection limits as low as 4.8 ng/mL. Penicillium pedernalense extract achieved a cGP content of 67.45 ± 1.11 ng/mL, with a corresponding fermentation yield of 29.31 ± 0.61 mg/L. This surpassed Penicillium steckii, which reached a content of 31.71 ± 0.31 ng/mL, with a yield of 8.51 ± 0.15 mg/L. This quantitative approach for metabolite analysis provides a viable method for screening these fungal strains, highlighting their potential for sustainable production of cyclic glycine-proline (cGP).

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来源期刊

Journal of Fungi Medicine-Microbiology (medical)

CiteScore

6.70

自引率

14.90%

发文量

1151

审稿时长

11 weeks

期刊介绍： Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.