NON-RIPENING 天然变体可促进西瓜果实成熟。

IF 10 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Cell Pub Date : 2024-11-27 DOI:10.1093/plcell/koae313
Jinfang Wang, Yongtao Yu, Shaogui Guo, Jie Zhang, Yi Ren, Shouwei Tian, Maoying Li, Shengjin Liao, Guoyi Gong, Haiying Zhang, Yong Xu
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引用次数: 0

摘要

转录因子 NON-RIPENING(NOR)对非周期性果实成熟的调控尚不十分清楚。在这里,我们发现非橙色果实西瓜(Citrullus lanatus)中的 NOR 同源物位于选择性横扫和甜度数量性状位点内,该位点在从陆地品种到栽培品种的驯化过程中被选择。与野生型相比,ClNOR基因敲除大大延迟了果实成熟,而且基因敲除植株的果实脱落酸(ABA)水平较低,果肉颜色较浅,甜度较低。转录组分析和 DNA 亲和纯化测序显示,ClNOR 以基本亮氨酸拉链基因 ClbZIP1 为靶标,它将 ClNOR 与启动子中没有 ClNOR 结合基序的基因联系起来,如 ABA 生物合成基因、9-顺式环氧类胡萝卜素二氧合酶 ClNCED1 和染色体磷酸盐转运基因 ClPHT4;2。双突变体 Clnor Clbzip1 表现出果实成熟延迟、ABA 水平降低和果肉颜色变浅。其延迟成熟表型比 Clbzip1 单突变体更强。此外,栽培西瓜中的 ClNORT,T 单倍型会导致 ClbZIP1 表达较高,但来自陆地品种的 ClNORC,T 和来自祖先西瓜的 ClNORC,G 则不会。异源 ClNORT,T 表达可挽救番茄(Solanum lycopersicum)中 Slnor 基因敲除的延迟成熟表型。ClNOR 的这种天然变体(564T/C)通过增强靶基因转录促进果实成熟。总之,这些发现将有助于阐明非橙色果实成熟的进化机制。
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A natural variant of NON-RIPENING promotes fruit ripening in watermelon.

The regulation of non-climacteric fruit ripening by the transcription factor NON-RIPENING (NOR) is poorly understood. Here, we identified that the NOR homolog in the non-climacteric fruit watermelon (Citrullus lanatus) is located within the selective sweep and sweetness quantitative trait locus that was selected during domestication from landraces to cultivars. ClNOR knockout substantially delayed fruit ripening, and the fruits of the knockout plants had lower abscisic acid (ABA) levels, lighter colored flesh, and were less sweet compared to wild type. Transcriptome analysis and DNA affinity purification sequencing revealed that ClNOR targeted the Basic Leucine Zipper gene ClbZIP1, which links ClNOR to genes that do not have a ClNOR-binding motif in their promoters, such as the ABA biosynthesis gene, 9-cis-epoxycarotenoid dioxygenase ClNCED1 and the chromoplast phosphate transporter gene ClPHT4;2. The double mutant Clnor Clbzip1 exhibited delayed fruit ripening, lower ABA levels, and lighter colored flesh. Its delayed ripening phenotype was stronger than that of the Clbzip1 single mutant. Additionally, the ClNORT,T haplotype in cultivated watermelon resulted in higher ClbZIP1 expression, but ClNORC,T from landraces and ClNORC,G from ancestral watermelon did not. Heterologous ClNORT,T expression rescued the delayed ripening phenotype of the Slnor knockout in tomato (Solanum lycopersicum). This natural variant (564T/C) of ClNOR promoted fruit ripening by enhancing target gene transcription. Overall, these findings will help elucidate the evolutionary mechanisms of non-climacteric fruit ripening.

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来源期刊
Plant Cell
Plant Cell 生物-生化与分子生物学
CiteScore
16.90
自引率
5.20%
发文量
337
审稿时长
2.4 months
期刊介绍: Title: Plant Cell Publisher: Published monthly by the American Society of Plant Biologists (ASPB) Produced by Sheridan Journal Services, Waterbury, VT History and Impact: Established in 1989 Within three years of publication, ranked first in impact among journals in plant sciences Maintains high standard of excellence Scope: Publishes novel research of special significance in plant biology Focus areas include cellular biology, molecular biology, biochemistry, genetics, development, and evolution Primary criteria: articles provide new insight of broad interest to plant biologists and are suitable for a wide audience Tenets: Publish the most exciting, cutting-edge research in plant cellular and molecular biology Provide rapid turnaround time for reviewing and publishing research papers Ensure highest quality reproduction of data Feature interactive format for commentaries, opinion pieces, and exchange of information in review articles, meeting reports, and insightful overviews.
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