Diosgenin Mediated JAK/STAT Signal Channel Improving SD Rat Renal Ischemia-Reperfusion

Objective

To explore the mechanism of action of diosgenin and JAK/STAT in SD rat renal ischemia-reperfusion (I/R) injury.

Method

A renal I/R injury model was established, rat was divided into four groups: control group, sham surgery group, I/R model group, and diosgenin pretreatment group. 48 hours after the completion of modeling, blood was collected for testing of SCr, BUN, and Kim-1. After blood collection, bilateral kidneys were cut and stored in liquid nitrogen for HE staining. Western blot was used to detect the protein expression levels of JAK and STAT in each group. Compare and analyze the differences in JAK/STAT expression levels among different groups.

Result

Compared with the I/R model group, the Scr, Bun, and Kim-1 concentrations of the three treatment groups were significantly lower, the other three treatment groups showed significant improvement in symptoms of renal vacuolar degeneration and interstitial edema in rats, the expression levels of JAK and STAT in the three groups were significantly lower, while the difference between the three groups was not significant.