约束应激致咬肌过动时CeA-Vme投射的调节。

IF 4.2 3区 医学 Q2 NEUROSCIENCES Frontiers in Cellular Neuroscience Pub Date : 2024-11-21 eCollection Date: 2024-01-01 DOI:10.3389/fncel.2024.1509020
Ya-Juan Zhao, Ji Chen, Yang Liu, Lv-La Pan, Yan-Xia Guo, Zhou-Ming Zhang, Qiang Li, Yong-Jin Chen
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引用次数: 0

摘要

咀嚼肌肉的过度活动(磨牙或咬牙)与压力暴露有关,并经常导致持续的肌肉疼痛。然而,其背后的神经机制尚不完全清楚。中央杏仁核(CeA)与压力诱发的行为和身体反应有关,它直接投射到中脑三叉神经核(Vme),而中脑三叉神经核对口腔运动协调至关重要。因此,我们假设从CeA到Vme的投影可能与压力引起的焦虑和下颌肌肉的过度活动有关。通过建立约束应激动物模型,我们发现慢性应激可导致明显的焦虑相关行为,咬肌活动增加,CeA中gabaergy神经元激活,而Vme中多极gabaergy中间神经元和伪单极兴奋神经元的兴奋性发生相反的变化。随后,通过顺行和跨突触示踪结合免疫荧光染色,我们发现从CeA到Vme的神经投射主要是gaba能的,并且CeA的投射终止于Vme内gaba能的中间神经元。此外,从化学上抑制CeA中gaba能神经元的功能可以有效降低焦虑水平,逆转咬肌因压力引起的活动增加。并且,通过光遗传学特异性地抑制从CeA到Vme的gaba能投射可以减少咬肌的过度活跃,但不能减少压力引起的焦虑。总之,我们的研究结果表明,从CeA到Vme的gaba能投射可能在慢性应激下咬肌过度活动中起重要作用。
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Regulation of CeA-Vme projection in masseter hyperactivity caused by restraint stress.

The overactivity of the masticatory muscles (bruxism or teeth clenching) is associated with stress exposure, and often leading to consistent muscle pain. However, the neural mechanism underlining it is not fully understood. The central amygdala (CeA), which is linked to stress-induced behaviors and physical reactions, projects directly to the mesencephalic trigeminal nucleus (Vme), which is crucial for oral-motor coordination. Thus, we hypothesized that the projections from the CeA to the Vme could be linked to stress-induced anxiety and overactivity of the jaw muscles. After establishing an animal model of restraint stress, we found that chronic stress could lead to noticeable anxiety-related behavior, increased masseter muscle activity, activation of GABAergic neurons in the CeA, and opposite changes in the excitability of multipolar GABAergic interneurons and pseudounipolar excitatory neurons in the Vme. Subsequently, through the utilization of anterograde and transsynaptic tracing in conjunction with immunofluorescence staining, we discovered that the neural projections from the CeA to the Vme were mainly GABAergic and that the projections from the CeA terminated on GABAergic interneurons within the Vme. Moreover, chemogenetically suppressing the function of GABAergic neurons in the CeA could effectively reduce anxiety levels and reverse the increase in the activity of the masseter muscles induced by stress. And, specifically inhibiting GABAergic projections from the CeA to the Vme via optogenetics could reduce the hyperactivity of the masseter muscles but not stress-induced anxiety. In conclusion, our findings indicate that GABAergic projections from the CeA to the Vme may play an important role in the masseter overactivity in response to chronic stress.

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来源期刊
CiteScore
7.90
自引率
3.80%
发文量
627
审稿时长
6-12 weeks
期刊介绍: Frontiers in Cellular Neuroscience is a leading journal in its field, publishing rigorously peer-reviewed research that advances our understanding of the cellular mechanisms underlying cell function in the nervous system across all species. Specialty Chief Editors Egidio D‘Angelo at the University of Pavia and Christian Hansel at the University of Chicago are supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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