The influence of sex and age differences in an adult zebrafish (Danio rerio) T-maze model of cognition.

The zebrafish (Danio rerio) model is increasingly popular in neurobehavioral research, and behavioral outcomes are commonly evaluated in studies on neurodegeneration and neurotoxicity. Sex and age have been identified as important variables in cognition studies; however, these factors are often underreported in published studies that use the zebrafish model, leading to uncertainty about their impact in zebrafish T-maze experiments. In this study, we evaluated the role of sex and age in zebrafish cognitive function using a 5-day T-maze task. Our results demonstrated that female and younger zebrafish had increased learning and memory capacity. These findings highlighted the importance of considering and reporting sex and age in experimental design in zebrafish cognitive neurobehavioral studies.