{"title":"使用放射性标记的2-脱氧-d -葡萄糖测量小鼠组织中葡萄糖利用的方案。","authors":"Cyun-Ming Wu, Hao-Yun Li, Wen-Yi Li, Chih-Neng Hsu, Wei-Shun Yang, Gwo-Tsang Chuang, Tony Pan-Hou Che, Tung-Yuan Lee, Hsiao-Lin Lee, Siow-Wey Hee, Jiin-Horng Lee, Daniel Liao, Karen Chia-Wen Liao, Ya-Wen Liu, Chin-Feng Hsuan, Yi-Cheng Chang, Shao-Lun Chu","doi":"10.1016/j.xpro.2024.103478","DOIUrl":null,"url":null,"abstract":"<p><p>2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103478"},"PeriodicalIF":1.3000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656071/pdf/","citationCount":"0","resultStr":"{\"title\":\"Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose.\",\"authors\":\"Cyun-Ming Wu, Hao-Yun Li, Wen-Yi Li, Chih-Neng Hsu, Wei-Shun Yang, Gwo-Tsang Chuang, Tony Pan-Hou Che, Tung-Yuan Lee, Hsiao-Lin Lee, Siow-Wey Hee, Jiin-Horng Lee, Daniel Liao, Karen Chia-Wen Liao, Ya-Wen Liu, Chin-Feng Hsuan, Yi-Cheng Chang, Shao-Lun Chu\",\"doi\":\"10.1016/j.xpro.2024.103478\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.</p>\",\"PeriodicalId\":34214,\"journal\":{\"name\":\"STAR Protocols\",\"volume\":\"5 4\",\"pages\":\"103478\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656071/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"STAR Protocols\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.xpro.2024.103478\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/6 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2024.103478","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/6 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose.
2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.
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