槲皮素对不同直径球形金纳米颗粒(GNPs)心脏毒性的保护作用。

IF 2.2 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Current pharmaceutical biotechnology Pub Date : 2024-12-10 DOI:10.2174/0113892010359481241122073753
Wael A M Ghonimi, Foll Alnada A F Abdelrahman, Fawiziah Khalaf Alharbi, Maha Alsunbul, Fawziah A Al-Salmi, Doaa Abdelrahaman, Eman Fayad, Shafika A El Sayed
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引用次数: 0

摘要

背景:金纳米粒子(GNPs)由于其独特的电学、力学、化学和光学特性,在生物医学领域引起了极大的关注。这些显著的电位与生物组织的相互作用具有毒性的风险。槲皮素(Qur)是一种天然类黄酮,具有多种药理作用,特别是抗炎、抗细胞凋亡和抗氧化。目的:研究不同直径的球形GNPs对心脏的潜在毒性,并评价其对毒性最大直径的GNPs可能的心脏保护作用。方法:将大鼠随机分组,分别给予或不给予球形GNPs(10、20、50 nm)和Qur (200 mg/kg b.wt)。采集心脏和血液标本,进行组织学、免疫组织化学和生化检查。结果:与20和50 nm处理组相比,10 nm GNPs显著提高了心肌生物标志物的水平,包括肌钙蛋白I、肌酸激酶同工酶mb (CK-MB)、CK-Total、乳酸脱氢酶(LDH)。组织病理学上,10 nm GNPs表现为严重的心肌细胞变性、萎缩、心肌纤维紊乱、局灶性出血、血管充血和间质炎性细胞浸润。免疫组织化学结果显示,10 nm GNPs对抗caspase-3抗体表现出强烈的阳性表达,证实心肌细胞广泛凋亡。然而,大多数这些病理改变在Qur治疗后明显改善。结论:GNPs的大小对其对心脏组织的毒理学影响至关重要,10 nm的GNPs比大颗粒的GNPs更能引起严重的组织损伤、强的细胞毒性和凋亡。此外,与Qur预处理显示了对GNPs心脏毒性的显着心脏保护作用。
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Cardioprotective Efficacy of Quercetin against Cardiotoxicity Induced by Different Diameters of Sphere Gold Nanoparticles (GNPs).

Background: Gold nanoparticles (GNPs) have garnered significant attention in the biomedicine field due to their exceptional electrical, mechanical, chemical, and optical characteristics. The interaction of these remarkable potentials with biological tissues carries a risk of toxicity. Quercetin (Qur) is a natural flavonoid and exhibits numerous pharmacological impacts, especially anti-inflammatory, anti-apoptotic, and antioxidant.

Objective: This investigation aimed to clarify the potential cardiotoxicity induced by different diameters of spherical GNPs as well as to evaluate the possible cardioprotective roles of Qur against the most toxic diameter of GNPs.

Methods: Rats were randomly grouped and treated with or without sphere GNPs (10, 20 and 50 nm) and Qur (200 mg/kg b.wt.). Heart and blood samples were collected and subjected to histological, immunohistochemical and biochemical investigations.

Results: When compared to the groups treated with 20 and 50 nm, the 10 nm GNPs dramatically increased the levels of cardiac biomarkers, including Troponin I, Creatine kinase isoenzyme-MB (CK-MB), CK-Total, lactate dehydrogenase (LDH). Histopathologically, 10 nm GNPs exhibited severe cardiomyocytes degenerations, atrophy, disorganization of myocardial fibers, focal hemorrhage, congested blood vessels and interstitial inflammatory cells infiltrations. Immunohistochemically, 10 nm GNPs exhibited strongly positive expressions against anti-caspase-3 antibody confirming extensive apoptosis of cardiomyocytes. However, the majority of these pathological changes were significantly improved upon Qur treatment.

Conclusion: The size of GNPs is crucial to their toxicological impact on cardiac tissues where 10 nm GNPs can induce severe histological damage, potent cytotoxicity, and apoptosis rather than larger particles. Otherwise, pre-co-treatment with Qur revealed a significant cardioprotective effect against GNPs cardiotoxicity.

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来源期刊
Current pharmaceutical biotechnology
Current pharmaceutical biotechnology 医学-生化与分子生物学
CiteScore
5.60
自引率
3.60%
发文量
203
审稿时长
6 months
期刊介绍: Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal includes timely in-depth reviews, original research articles and letters written by leaders in the field, covering a range of current topics in scientific areas of Pharmaceutical Biotechnology. Invited and unsolicited review articles are welcome. The journal encourages contributions describing research at the interface of drug discovery and pharmacological applications, involving in vitro investigations and pre-clinical or clinical studies. Scientific areas within the scope of the journal include pharmaceutical chemistry, biochemistry and genetics, molecular and cellular biology, and polymer and materials sciences as they relate to pharmaceutical science and biotechnology. In addition, the journal also considers comprehensive studies and research advances pertaining food chemistry with pharmaceutical implication. Areas of interest include: DNA/protein engineering and processing Synthetic biotechnology Omics (genomics, proteomics, metabolomics and systems biology) Therapeutic biotechnology (gene therapy, peptide inhibitors, enzymes) Drug delivery and targeting Nanobiotechnology Molecular pharmaceutics and molecular pharmacology Analytical biotechnology (biosensing, advanced technology for detection of bioanalytes) Pharmacokinetics and pharmacodynamics Applied Microbiology Bioinformatics (computational biopharmaceutics and modeling) Environmental biotechnology Regenerative medicine (stem cells, tissue engineering and biomaterials) Translational immunology (cell therapies, antibody engineering, xenotransplantation) Industrial bioprocesses for drug production and development Biosafety Biotech ethics Special Issues devoted to crucial topics, providing the latest comprehensive information on cutting-edge areas of research and technological advances, are welcome. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.
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