CRISPR解锁了保持其大小的甜番茄

IF 33.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Nature biotechnology Pub Date : 2024-12-11 DOI:10.1038/s41587-024-02510-x
Iris Marchal
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引用次数: 0

摘要

番茄(Solanum lycopersicum)的驯化导致了果实尺寸的大幅增加,但代价是糖含量的降低,这种负相关可能是高糖等位基因丢失的结果。在《自然》杂志上发表的一项研究中,Zhang等人通过突变编码一种调节糖积累的蛋白质的基因,培育出一种甜番茄,使其保持高产和重。Zhang等人对野生和栽培番茄进行了全基因组关联研究,以寻找与果实甜度相关的基因组区域。这项分析确定了六个基因,其中只有一个在甜番茄中表达水平较低。该基因被称为SlCDPK27及其旁系物SlCDPK26,其功能表征表明,它们编码的激酶在果实成熟过程中表达增加,在果实成熟阶段,它们通过磷酸化蔗糖合酶SlSUS3(促进该酶的降解)来降低糖含量。作者表明,crispr - cas9介导的SlCDPK27和SlCDPK26的双敲除使一种常用番茄品种的糖含量增加了30%,而不影响果实大小。
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来源期刊
Nature biotechnology
Nature biotechnology 工程技术-生物工程与应用微生物
CiteScore
63.00
自引率
1.70%
发文量
382
审稿时长
3 months
期刊介绍: Nature Biotechnology is a monthly journal that focuses on the science and business of biotechnology. It covers a wide range of topics including technology/methodology advancements in the biological, biomedical, agricultural, and environmental sciences. The journal also explores the commercial, political, ethical, legal, and societal aspects of this research. The journal serves researchers by providing peer-reviewed research papers in the field of biotechnology. It also serves the business community by delivering news about research developments. This approach ensures that both the scientific and business communities are well-informed and able to stay up-to-date on the latest advancements and opportunities in the field. Some key areas of interest in which the journal actively seeks research papers include molecular engineering of nucleic acids and proteins, molecular therapy, large-scale biology, computational biology, regenerative medicine, imaging technology, analytical biotechnology, applied immunology, food and agricultural biotechnology, and environmental biotechnology. In summary, Nature Biotechnology is a comprehensive journal that covers both the scientific and business aspects of biotechnology. It strives to provide researchers with valuable research papers and news while also delivering important scientific advancements to the business community.
期刊最新文献
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