Enhancing medicinal proteins production in plant bioreactors: A focal review on promoters.

The use of plant bioreactors for the production of medicinal proteins has emerged as a promising and cost-effective alternative to traditional microbial and mammalian cell culture systems. This review provides a focused examination of the critical role of promoters in enhancing the production of therapeutic proteins within plant-based platforms. We discuss the latest advancements in promoter discovery, modification, and optimization for the expression of medicinal proteins in plants. The review highlights the challenges and opportunities associated with various types of promoters, including constitutive, tissue-specific, and inducible promoters, and their impact on medicinal protein yield and quality. Case studies are presented to illustrate the successful application of these promoter engineering techniques in plant bioreactors, emphasizing the potential for scalable and sustainable production of pharmaceutical proteins. Additionally, we explore the strategies for improving promoter function. This review is intended to guide researchers and industry professionals in the selection and design of promoters for the enhanced production of medicinal proteins in plant bioreactors.