{"title":"敲除LncRNA-GAS5恢复鲍曼不动杆菌对Cuprotosis蛋白FDX-1的抑制","authors":"Zhiyuan An, Wenyi Ding","doi":"10.33073/pjm-2024-040","DOIUrl":null,"url":null,"abstract":"<p><p>This study investigated the regulatory effect of LncRNA-GAS5 on FDX-1 in HEK293T cells by <i>Acinetobacter baumannii</i>. Transfected LncRNA-GAS5 overexpressing or knocking down plasmids into HEK293T cells, and the expression of FDX-1 was detected by Western blotting. <i>A. baumannii</i> inhibited the expression of FDX-1. Overexpression of LncRNA-GAS5 inhibited the expression of FDX-1, while knocking down LncRNA-GAS5 increased the expression of FDX-1. Overexpression of LncRNA-GAS5 further enhanced the inhibitory effect of <i>A. baumannii</i> on FDX-1, while knocking down LncRNA-GAS5 restored the inhibitory effect of <i>A. baumannii</i> on FDX-1. LncRNA-GAS5 regulates the inhibitory effect of <i>A. baumannii</i> on FDX-1 in HEK293T cells.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"73 4","pages":"535-539"},"PeriodicalIF":1.6000,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639367/pdf/","citationCount":"0","resultStr":"{\"title\":\"Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by <i>Acinetobacter baumannii</i>.\",\"authors\":\"Zhiyuan An, Wenyi Ding\",\"doi\":\"10.33073/pjm-2024-040\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study investigated the regulatory effect of LncRNA-GAS5 on FDX-1 in HEK293T cells by <i>Acinetobacter baumannii</i>. Transfected LncRNA-GAS5 overexpressing or knocking down plasmids into HEK293T cells, and the expression of FDX-1 was detected by Western blotting. <i>A. baumannii</i> inhibited the expression of FDX-1. Overexpression of LncRNA-GAS5 inhibited the expression of FDX-1, while knocking down LncRNA-GAS5 increased the expression of FDX-1. Overexpression of LncRNA-GAS5 further enhanced the inhibitory effect of <i>A. baumannii</i> on FDX-1, while knocking down LncRNA-GAS5 restored the inhibitory effect of <i>A. baumannii</i> on FDX-1. LncRNA-GAS5 regulates the inhibitory effect of <i>A. baumannii</i> on FDX-1 in HEK293T cells.</p>\",\"PeriodicalId\":94173,\"journal\":{\"name\":\"Polish journal of microbiology\",\"volume\":\"73 4\",\"pages\":\"535-539\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-12-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11639367/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33073/pjm-2024-040\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/12/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33073/pjm-2024-040","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Knocking Down LncRNA-GAS5 Restores the Inhibition of Cuprotosis Protein FDX-1 by Acinetobacter baumannii.
This study investigated the regulatory effect of LncRNA-GAS5 on FDX-1 in HEK293T cells by Acinetobacter baumannii. Transfected LncRNA-GAS5 overexpressing or knocking down plasmids into HEK293T cells, and the expression of FDX-1 was detected by Western blotting. A. baumannii inhibited the expression of FDX-1. Overexpression of LncRNA-GAS5 inhibited the expression of FDX-1, while knocking down LncRNA-GAS5 increased the expression of FDX-1. Overexpression of LncRNA-GAS5 further enhanced the inhibitory effect of A. baumannii on FDX-1, while knocking down LncRNA-GAS5 restored the inhibitory effect of A. baumannii on FDX-1. LncRNA-GAS5 regulates the inhibitory effect of A. baumannii on FDX-1 in HEK293T cells.
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