{"title":"乙烯反应转录因子5（ERF5）可增强烟草卷曲芽病毒及相关β卫星（TbCSV/TbCSB）在烟草中的防御能力。","authors":"Meisheng Zhao , Liping Zhang , Hussein Ghanem , Gentu Wu , Mingjun Li , Ling Qing","doi":"10.1016/j.virol.2024.110309","DOIUrl":null,"url":null,"abstract":"<div><div>Begomovirus/betasatellite disease complex significantly threatens global crop production. Identifying potential plant antiviral genes is crucial for disease control. <em>Nicotiana benthamiana</em> is susceptible to viruses and contains 266 ethylene response transcription factors (ERFs). This study identified 29 <em>NbERF</em>s that were differentially upregulated in tobacco curly shoot virus and its associated betasatellite (TbCSV/TbCSB) infection, with ERF5 being the most common. Nine NbERF5s cluster phylogenetically and <em>Niben101Scf00163g22002</em> (<em>NbERF5</em>) responds significantly to exogenous ACC treatment. Further analysis confirms the nuclear localization and transcriptional activation activity of NbERF5. Protein interaction assays demonstrate that NbERF5 has no self-interaction and does not interact with the βC1 protein of TbCSB. Silencing <em>NbERF5</em> enhances TbCSV/TbCSB infection, and overexpression of <em>NbERF5</em> inhibits TbCSV/TbCSB infection. Importantly, <em>NbERF5</em> positively regulates the expression of the pathogenesis-related (<em>PR</em>) genes, <em>NbPR1a</em> and <em>NbNPR1</em>. Our findings suggest that <em>NbERF5</em> enhances TbCSV/TbCSB resistance by activating the <em>PR</em> genes, indicating that <em>NbERF5</em> is a potential antiviral gene.</div></div>","PeriodicalId":23666,"journal":{"name":"Virology","volume":"603 ","pages":"Article 110309"},"PeriodicalIF":2.8000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ethylene response transcription factor 5 (ERF5) enhances defense against tobacco curly shoot virus and associated betasatellite (TbCSV/TbCSB) in Nicotiana benthamiana\",\"authors\":\"Meisheng Zhao , Liping Zhang , Hussein Ghanem , Gentu Wu , Mingjun Li , Ling Qing\",\"doi\":\"10.1016/j.virol.2024.110309\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Begomovirus/betasatellite disease complex significantly threatens global crop production. Identifying potential plant antiviral genes is crucial for disease control. <em>Nicotiana benthamiana</em> is susceptible to viruses and contains 266 ethylene response transcription factors (ERFs). This study identified 29 <em>NbERF</em>s that were differentially upregulated in tobacco curly shoot virus and its associated betasatellite (TbCSV/TbCSB) infection, with ERF5 being the most common. Nine NbERF5s cluster phylogenetically and <em>Niben101Scf00163g22002</em> (<em>NbERF5</em>) responds significantly to exogenous ACC treatment. Further analysis confirms the nuclear localization and transcriptional activation activity of NbERF5. Protein interaction assays demonstrate that NbERF5 has no self-interaction and does not interact with the βC1 protein of TbCSB. Silencing <em>NbERF5</em> enhances TbCSV/TbCSB infection, and overexpression of <em>NbERF5</em> inhibits TbCSV/TbCSB infection. Importantly, <em>NbERF5</em> positively regulates the expression of the pathogenesis-related (<em>PR</em>) genes, <em>NbPR1a</em> and <em>NbNPR1</em>. Our findings suggest that <em>NbERF5</em> enhances TbCSV/TbCSB resistance by activating the <em>PR</em> genes, indicating that <em>NbERF5</em> is a potential antiviral gene.</div></div>\",\"PeriodicalId\":23666,\"journal\":{\"name\":\"Virology\",\"volume\":\"603 \",\"pages\":\"Article 110309\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Virology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0042682224003337\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0042682224003337","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
Ethylene response transcription factor 5 (ERF5) enhances defense against tobacco curly shoot virus and associated betasatellite (TbCSV/TbCSB) in Nicotiana benthamiana
Begomovirus/betasatellite disease complex significantly threatens global crop production. Identifying potential plant antiviral genes is crucial for disease control. Nicotiana benthamiana is susceptible to viruses and contains 266 ethylene response transcription factors (ERFs). This study identified 29 NbERFs that were differentially upregulated in tobacco curly shoot virus and its associated betasatellite (TbCSV/TbCSB) infection, with ERF5 being the most common. Nine NbERF5s cluster phylogenetically and Niben101Scf00163g22002 (NbERF5) responds significantly to exogenous ACC treatment. Further analysis confirms the nuclear localization and transcriptional activation activity of NbERF5. Protein interaction assays demonstrate that NbERF5 has no self-interaction and does not interact with the βC1 protein of TbCSB. Silencing NbERF5 enhances TbCSV/TbCSB infection, and overexpression of NbERF5 inhibits TbCSV/TbCSB infection. Importantly, NbERF5 positively regulates the expression of the pathogenesis-related (PR) genes, NbPR1a and NbNPR1. Our findings suggest that NbERF5 enhances TbCSV/TbCSB resistance by activating the PR genes, indicating that NbERF5 is a potential antiviral gene.
期刊介绍：
Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.
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