R. Eustáquio, A. T. Caldeira, S. Arantes, A. Candeias, A. Pereira
{"title":"新的4-苯乙烯香豆素衍生物作为生物分子的潜在荧光标记:在rna -鱼探针中的应用","authors":"R. Eustáquio, A. T. Caldeira, S. Arantes, A. Candeias, A. Pereira","doi":"10.1134/S000368382360286X","DOIUrl":null,"url":null,"abstract":"<p>Fluorescence microscopy is a highly sensitive imaging technique used in various scientific fields such as cellular biology, environmental sciences, medicine, and pharmacy. It offers the advantage of using multiple fluorescent labels to visualize different biomolecules and generate multicolored images for identifying specific components within complex biomolecular structures and studying their interactions. These fluorescent labels create chemically stable and minimally disruptive bioconjugates. Amino-reactive fluorescent labels, due to their ease of incorporation into biomolecules, are commonly used in applications like fluorescence in situ hybridization, histochemistry, cell tracing, receptor binding, and immunochemistry. However, the existing popular fluorescent labels are expensive, making coumarin derivatives a potential cost-effective solution for developing bright fluorophores. In this study, the fluorescent 4-styrylcoumarin derivative labels were synthesized and evaluated as potentially effective fluorescent labels for biomolecules. Twelve new fluorescent oligonucleotide probes have been obtained, 6 directed to the rRNA region of eukaryotic cells (EUK516) and 6 directed to the rRNA region of prokaryotic cells (EUB338). The developed fluorescent probes were tested on microorganisms belonging to the culture collection of the Laboratory of Biodegradation and Biotechnology of the HERCULES Laboratory (University of Évora, Portugal), showing effective performance as RNA-FISH probes. These findings evidenced the applicability of the new 4-styrylcoumarin derivatives in labeling of biomolecules and bioimaging.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1118 - 1130"},"PeriodicalIF":1.0000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"New 4-styrylcoumarin Derivatives as Potentials Fluorescent Labels for Biomolecules: Application in RNA-fish Probes\",\"authors\":\"R. Eustáquio, A. T. Caldeira, S. Arantes, A. Candeias, A. Pereira\",\"doi\":\"10.1134/S000368382360286X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Fluorescence microscopy is a highly sensitive imaging technique used in various scientific fields such as cellular biology, environmental sciences, medicine, and pharmacy. It offers the advantage of using multiple fluorescent labels to visualize different biomolecules and generate multicolored images for identifying specific components within complex biomolecular structures and studying their interactions. These fluorescent labels create chemically stable and minimally disruptive bioconjugates. Amino-reactive fluorescent labels, due to their ease of incorporation into biomolecules, are commonly used in applications like fluorescence in situ hybridization, histochemistry, cell tracing, receptor binding, and immunochemistry. However, the existing popular fluorescent labels are expensive, making coumarin derivatives a potential cost-effective solution for developing bright fluorophores. In this study, the fluorescent 4-styrylcoumarin derivative labels were synthesized and evaluated as potentially effective fluorescent labels for biomolecules. Twelve new fluorescent oligonucleotide probes have been obtained, 6 directed to the rRNA region of eukaryotic cells (EUK516) and 6 directed to the rRNA region of prokaryotic cells (EUB338). The developed fluorescent probes were tested on microorganisms belonging to the culture collection of the Laboratory of Biodegradation and Biotechnology of the HERCULES Laboratory (University of Évora, Portugal), showing effective performance as RNA-FISH probes. These findings evidenced the applicability of the new 4-styrylcoumarin derivatives in labeling of biomolecules and bioimaging.</p>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"60 6\",\"pages\":\"1118 - 1130\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S000368382360286X\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S000368382360286X","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
New 4-styrylcoumarin Derivatives as Potentials Fluorescent Labels for Biomolecules: Application in RNA-fish Probes
Fluorescence microscopy is a highly sensitive imaging technique used in various scientific fields such as cellular biology, environmental sciences, medicine, and pharmacy. It offers the advantage of using multiple fluorescent labels to visualize different biomolecules and generate multicolored images for identifying specific components within complex biomolecular structures and studying their interactions. These fluorescent labels create chemically stable and minimally disruptive bioconjugates. Amino-reactive fluorescent labels, due to their ease of incorporation into biomolecules, are commonly used in applications like fluorescence in situ hybridization, histochemistry, cell tracing, receptor binding, and immunochemistry. However, the existing popular fluorescent labels are expensive, making coumarin derivatives a potential cost-effective solution for developing bright fluorophores. In this study, the fluorescent 4-styrylcoumarin derivative labels were synthesized and evaluated as potentially effective fluorescent labels for biomolecules. Twelve new fluorescent oligonucleotide probes have been obtained, 6 directed to the rRNA region of eukaryotic cells (EUK516) and 6 directed to the rRNA region of prokaryotic cells (EUB338). The developed fluorescent probes were tested on microorganisms belonging to the culture collection of the Laboratory of Biodegradation and Biotechnology of the HERCULES Laboratory (University of Évora, Portugal), showing effective performance as RNA-FISH probes. These findings evidenced the applicability of the new 4-styrylcoumarin derivatives in labeling of biomolecules and bioimaging.
期刊介绍:
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.