辐射诱导海星卵母细胞的旁观者效应。

IF 2.5 3区医学 Q2 BIOLOGY Radiation research Pub Date : 2025-01-01 DOI:10.1667/RADE-23-00198.1

Haruki Chiba, Hinata Horikawa, Valerie Swee Ting Goh, Tomisato Miura, Kentaro Ariyoshi

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引用次数: 0

摘要

尽管多项研究表明，电离辐射可在培养细胞系中诱导旁观者效应（radiation-induced bystander effect， RIBE），但RIBE在无脊椎动物中是否具有进化保守性尚不清楚。在本研究中，我们研究了未辐照海星（Patiria pectinifera）卵母细胞与X射线（0、2和4 Gy）照射的卵母细胞共培养的细胞死亡频率。我们观察到，未辐照的卵母细胞与2 Gy或4 Gy辐照的卵母细胞共培养时，细胞死亡的频率增加，由形态异常和tunel阳性细胞决定。此外，海水培养的4 Gy辐照卵母细胞诱导未辐照卵母细胞细胞死亡，tunel阳性细胞。我们的结果表明RIBE在海星中是进化保守的。

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Radiation-induced Bystander Effect in Starfish (Patiria pectinifera) Oocytes.

Although multiple studies suggest that ionizing radiation can induce bystander effects (radiation-induced bystander effect, RIBE) in cultured cell lines, it is still unclear whether RIBE is evolutionarily conserved in invertebrates. In this study, we investigated the frequency of cell death of unirradiated starfish (Patiria pectinifera) oocytes co-cultured with oocytes irradiated with X rays (0, 2 and 4 Gy). We observed increased frequencies of cell death determined by morphological abnormality and TUNEL-positive cells in unirradiated oocytes co-cultured with oocytes irradiated with 2 Gy or 4 Gy oocytes. In addition, the seawater cultured with 4 Gy irradiated oocytes induced cell death in unirradiated oocytes, and TUNEL-positive cells were observed. Our results suggest that RIBE is evolutionarily conserved in starfish.

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来源期刊

Radiation research 医学-核医学

CiteScore

5.10

自引率

8.80%

发文量

179

审稿时长

1 months

期刊介绍： Radiation Research publishes original articles dealing with radiation effects and related subjects in the areas of physics, chemistry, biology and medicine, including epidemiology and translational research. The term radiation is used in its broadest sense and includes specifically ionizing radiation and ultraviolet, visible and infrared light as well as microwaves, ultrasound and heat. Effects may be physical, chemical or biological. Related subjects include (but are not limited to) dosimetry methods and instrumentation, isotope techniques and studies with chemical agents contributing to the understanding of radiation effects.